A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene late
- PDF / 457,246 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 16 Downloads / 298 Views
ORIGINAL PAPER
A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres Xiao Liu 1,2,3 & Jifei Yang 2 & Qingmei Li 2 & Yinbiao Wang 4 & Yanhong Wang 2 & Ge Li 1 & Jianzhou Shi 2 & Peiyang Ding 5 & Junqing Guo 2 & Ruiguang Deng 2 & Gaiping Zhang 1,2 Received: 9 December 2019 / Accepted: 31 March 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The AuNP and PS particles were linked using monoclonal antibodies against influenza virus as the bridge. Under the optimal conditions, the visual detection limit of the AuNP-PSbased strip test was as low as 1/16 hemagglutination unit (HAU). It was 64 times higher than that of 10 nm (4 HAU) AuNP-based strip tests. Quantitative analysis showed that the detection limit of the AuNP-PS-based strip is 0.016 HAU. The AuNP-PS-based strip test showed no cross-reactivity to the other subtypes (H1, H5, H7, or H9) of influenza viruses. Keywords Lateral flow immunoassay . Nanocomposite . Influenza virus . Point-of-care test . Visual determination
Introduction Influenza virus causes worldwide flu epidemics each year [1, 2]. Methods for the detection of influenza virus include virus isolation, enzyme-linked immunosorbent assay (ELISA), and real-time reverse transcription polymerase chain reaction (RT-PCR). Virus isolation is timeconsuming and has low sensitivity. RT-PCR and ELISA
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04255-1) contains supplementary material, which is available to authorized users. * Gaiping Zhang [email protected] 1
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China
2
Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
3
Department of Medicine, Henan Medical College, Zhengzhou 451191, China
4
School of Public Health, Xinxiang Medical University, Xinxiang 453003, China
5
School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China
have improved sensitivity, but they also rely on professional operators and facilities [3–5]. Lateral flow strip tests (LFSTs) avoid the shortcomings of the above methods. LFSTs are simple, rapid, and specific and thus have been widely used in the point-of-care testing of diseases [6, 7], drug residues [8], and hormones [9]. Colloidal gold is one of the most common labels in LFSTs [10, 11]. The best colloidal gold-based LFST for detecting influenza (H3N2) virus has the detection limit of more than 103.5 TCID50· mL−1 (Table S1, 2) [12, 13]. Because early detection is necessary for prevention and control of influenza disease, several nanomaterial-based methods have been developed to increase sensitivity of the detection [14, 15]. Wiriyachaiporn et al. develop
Data Loading...
