A study of antigen-specific anti-cytomegalovirus antibody reactivity in patients with systemic sclerosis and concomitant
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Rheumatology International https://doi.org/10.1007/s00296-020-04643-z
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A study of antigen‑specific anti‑cytomegalovirus antibody reactivity in patients with systemic sclerosis and concomitant anti‑Ro52 antibodies Athanasios Gkoutzourelas1 · Christos Liaskos1 · Theodora Simopoulou1 · Christina Katsiari1 · George Efthymiou1 · Thomas Scheper2 · Wolfgang Meyer2 · Alexandra Tsirogianni3 · Christina Tsigalou4 · Efthymios Dardiotis5 · Dimitrios Daoussis6 · Lazaros I. Sakkas1 · Dimitrios P. Bogdanos1,2 Received: 11 January 2020 / Accepted: 3 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Anti-Ro52 autoantibody (autoAb), highly prevalent in Sjogren’s syndrome (SjS) and systemic lupus erythematosus (SLE), is also frequent in systemic sclerosis (SSc). Viral agents, such as human cytomegalovirus (HCMV), have been considered as a trigger for SSc and SSc-associated autoAbs. To seek for antigen-specific anti-HCMV associations with anti-Ro52, we assessed the dominant anti-HCMV ab responses in anti-Ro52 antibody (ab)-positive and -negative patients with SSc and compared them with those in SLE and SjS. 116 Anti-HCMV ab(+) sera were analyzed, including 70 from anti-Ro52(+) patients (29 SSc, 23 SLE and 18 SjS) and 46 from anti-Ro52(−) patients (29 with SSc, 9 with SLE and 8 with SjS) as negative controls. Abs against specific HCMV pp130/UL57, pp65/UL83, pp55/UL55, pp52/UL44, p38 and pp28/UL99 antigens were tested by immunoblotting. Anti-Ro52(+) SSc patients reacted more frequently against pp52/UL44 and p38 compared to anti-Ro52(−) [(13/29, 44.8%; 95% CI 26.7–62.9% vs. 1/29, 3.4%; 95% CI 0–10%, p
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