A Study on Molecular Mechanisms of Terahertz Radiation Interaction with Biopolymers Based on the Example of Bovine Serum
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CULAR BIOPHYSICS
A Study on Molecular Mechanisms of Terahertz Radiation Interaction with Biopolymers Based on the Example of Bovine Serum Albumin E. F. Nemovaa, c, *, O. P. Cherkasovaa, N. A. Nikolaeva, b, and G. G. Dultsevac aInstitute
of Laser Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia Institute of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia c Voevodsky Institute of Chemical Kinetics and Combustion, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia *e-mail: [email protected]
b
Received November 29, 2019; revised November 29, 2019; accepted February 17, 2020
Abstract—The effect of terahertz radiation on the functional activity of bovine serum albumin was studied by electron paramagnetic resonance spectroscopy using spin trapping and spin probing techniques. The use of a pH sensitive imidazoline spin trap allowed us to record the change in the binding strength of albumin with nitric oxide. The change in the intensity and width of bands in the EPR spectrum of the spin probe under terahertz irradiation was shown using a spin probe formed directly in the aqueous solution of albumin from a precursor, dihydropyrazine dioxide, which indicates a change in the number and mobility of paramagnetic centers. The observed changes were correlated with the structural characteristics of the reaction centers of bovine serum albumin, the functional groups of the amino acids of the protein. Keywords: terahertz radiation, bovine serum albumin, EPR spectroscopy, spin trap, spin probe, conformation DOI: 10.1134/S000635092003015X
INTRODUCTION Terahertz radiation covers the spectral range from 0.1 to 10 THz and lies between the microwave and infrared regions [1]. This radiation is absorbed by water; one of the first examples of biomedical applications of this radiation was associated with tracking changes in the water content in biological objects [2]. Biological effects of terahertz radiation have been studied at the cellular level [3] and at the body level [4]. Thus, for example, it was reported that terahertz radiation affects the change in the number of mutations in drosophila after the pretreatment with gamma radiation [5]. An effect of terahertz radiation on DNA of human leukocytes [6] and on fibroblasts [7] was found. The interest in studying biological effects of terahertz radiation is associated with the fact that the frequencies of vibrational–rotational transitions for many biological polymers lie in this spectral region. The energy of the terahertz radiation quantum is not large enough to break even weak hydrogen bonds; therefore, one cannot expect direct chemical action of the radiation in this range. The region of terahertz radiation energetically corresponds to collective vibraAbbreviations: BSA, bovine serum albumin; DPDO, 1,4-dihydropyrazine dioxide; EPR, electron paramagnetic resonance; PMIO, 1,2,2,5,5-pentamethyl-3-imidazoline-3-oxide.
tional–rotational transitions and changes in the conformatio
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