A spectroscopic insight into the interaction of chromene 1,2,4-oxadiazole-based compounds with bovine serum albumin
- PDF / 1,696,362 Bytes
- 15 Pages / 439.37 x 666.142 pts Page_size
- 22 Downloads / 173 Views
A spectroscopic insight into the interaction of chromene 1,2,4‑oxadiazole‑based compounds with bovine serum albumin Nilima Priyadarsini Mishra1 · Lakoji Satish1 · Seetaram Mohapatra1 · Sabita Nayak1 · Harekrushna Sahoo2 Received: 6 August 2020 / Accepted: 9 November 2020 © Springer Nature B.V. 2020
Abstract Four synthesized 2H-chromene-based 1,2,4-oxadiazole compounds (6a-6d) were studied for binding with bovine serum albumin (BSA) by different spectroscopic and thermodynamic analyses. A molecular docking program was used to find out the possible binding sites and binding affinity of 2H-chromene based 1,2,4-oxadiazole compounds with bovine serum albumin (BSA). The intrinsic fluorescence of BSA was quenched by these compounds through static quenching mechanism, and the estimated binding constant (Kb) value was found to be 1.5 × 103–10 × 103. The conformational changes of BSA were monitored by circular dichroism analysis, and it was observed that BSA is structurally stable in the presence of these compounds. The thermodynamic results indicated that the interaction process is spontaneous and the binding between oxadiazole compounds and BSA is mainly driven by hydrogen bonding and van der Waals forces. This study would be helpful to understand the biological benefits of oxadiazole-based compounds as well as the nature of interactions with biomolecules.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s1116 4-020-04323-4) contains supplementary material, which is available to authorized users. * Seetaram Mohapatra [email protected] 1
Department of Chemistry, Ravenshaw University, Cuttack 753003, India
2
Department of Chemistry, NIT Rourkela, Rourkela 769008, India
13
Vol.:(0123456789)
N. P. Mishra et al.
Graphic abstract
Keywords 1,2,4-oxadiazole · Microwave irradiation · Bovine serum albumin · Molecular docking · Circular dichroism
Introduction Heterocyclic molecules implanted with nitrogen and oxygen atoms have been used in diverse biological and pharmacological applications [1]. Among different types of nitrogen- and oxygen-containing heterocyclic compounds, five-membered heterocyclic compounds including azole, thiazole, oxadiazole, triazene, imidazole, purines, pyrazole, etc., play a very important role in human life due to their medicinal action against several diseases [2–9]. Specifically, oxadiazole-based compounds were found to exhibit anti-inflammatory, antimicrobial, antitubercular, antidepressant, antimalarial, anti-HIV and anticancer properties [10–19]. Due to their biological activities, synthesis of novel oxadiazole compounds is the current research area of interest to produce analgesics, antispasmodics and anti-inflammatories. Recently, our research group reported microwave-assisted synthesis of chromene-based 1,2,4-oxadiazole molecules having potent antibacterial activity [20]. Also, as reported earlier, these oxadiazole nuclei contribute significant bioactivity due to their lipophilic nature and have ability to bind with the
Data Loading...
