A thorough screening based on QTLs controlling zinc and copper accumulation in the grain of different wheat genotypes
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RESEARCH ARTICLE
A thorough screening based on QTLs controlling zinc and copper accumulation in the grain of different wheat genotypes Ying Liu 1 & Yaru Chen & Yang Yang 1 & Qiaofeng Zhang 2 & Bisheng Fu 2 & Jin Cai 2 & Wei Guo 2 & Liang Shi 1 & Jizhong Wu 2 & Yahua Chen 1 Received: 19 January 2020 / Accepted: 16 November 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Excess trace metals may cause damage to human health due to the consumption of food grain grown in contaminated soils. This study was designed to understand the genetic mechanisms of copper (Cu) and zinc (Zn) accumulation in wheat grain under stressed environments. The differences of Cu/Zn contents in the grain among 246 wheat varieties were analyzed, and the wheat varieties with low or high accumulation of Cu and Zn in the safe range were also screened out. The accumulation of Cu and Zn in grains of “Chushanbao” was lowest, which could be used as a novel germplasm for wheat breeding under heavy metal stress. We found that Cu contents of wheat grain were significantly and positively correlated with Zn. The quantitative trait loci (QTLs) for grain Cu content (GCuC) and grain Zn content (GZnC) were detected by genome-wide association study (GWAS). Twenty-three loci affecting GCuC were identified on chromosomes 1A, 1D, 2A, 2B, 2D, 3A, 3B, 3D, 4A, 4B 4D, 5A, 6D, 7A, and 7B, explaining 2.6–5.8% of the phenotypic variation. Sixteen loci associated with the GZnC on 11 different chromosomes 1B, 2B, 2D, 3A, 3D, 4A, 4B, 5A, 5D, 6B, and 7D were detected, which could explain 2.7~6.6% of phenotypic variance. We also determined five associated loci on chromosomes 2B, 2D, 3A, 4B, and 5A were in pleiotropic regions affecting both GCuC and GZnC. This study would help in better understanding the molecular basis of Cu/Zn accumulation in wheat grain, and the associated markers may be useful for marker-assisted selection (MAS) breeding program. Keywords Accumulation . Copper . Zinc . Phenotypic variation . QTL
Introduction
Ying Liu and Yaru Chen contributed equally to this work. Responsible Editor: Gangrong Shi * Liang Shi [email protected] * Jizhong Wu [email protected] 1
College of Life Sciences, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, National Joint Local Engineering Research Center for Rural Land Resources Use and Consolidation, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China
2
Provincial Key Laboratory of Agrobiology, The Jiangsu Provincial Infrastructure for Conservation and Utilization of Agricultural Germplasm, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, People’s Republic of China
Contamination of agricultural soil by heavy metals has become a detrimental environmental problem worldwide. Unlike organic pollutants, heavy metals can continuously accumulate in farm produce due to their non-biodegradable and persistent nature (Muhammad et al. 2011; Meena and Sarita 2019; Sarwar et al. 2010). Metal-polluted far
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