Abscisic acid in preservation of Taraxacum pieninicum in the form of synthetic seeds in slow growth conditions
- PDF / 2,226,598 Bytes
- 18 Pages / 595.276 x 790.866 pts Page_size
- 47 Downloads / 145 Views
ORIGINAL ARTICLE
Abscisic acid in preservation of Taraxacum pieninicum in the form of synthetic seeds in slow growth conditions Monika Kamińska1 · Jacek Kęsy2 · Alina Trejgell2 Received: 17 April 2020 / Accepted: 1 September 2020 © The Author(s) 2020
Abstract Taraxacum pieninicum Pawł. is listed as critically endangered species, for which currently applied protection methods are insufficient. The aim of this study was to investigate the possibility of T. pieninicum storage in the form of synthetic seeds under slow-growth conditions in combination with ABA treatment, as one of the ex situ protection methods of this species. The obtained results indicated that darkness was much more favorable condition for synseed storage and did not generate additional stress during cold exposure in contrast to the light conditions. The preculture of shoot tips on the medium supplemented with ABA led to a decrease in the shoots proliferation rate and inhibition of their growth. ABA clearly inhibited growth of the encapsulated shoot tips also during cold storage. Biochemical parameters showed that ABA effectively reduced the negative effect of the cold stress, what was found on the basis of analyzes of H2O2 and TBARS levels in the stored material. Moreover, synseeds stored under light conditions and treated with ABA exhibited decreased level of endogenous jasmonic acid what indicated interaction between those two phytohormones at a low temperature. The study also demonstrated that in vitro culture, cold storage and ABA treatment had no effect on the flowering process of this species after acclimatization to ex vitro conditions. Key message The aim of this research was to assess ABA effect on post-storage regrowth and the stress factors level in plants obtained after long-term storage of Taraxacum pieninicum synthetic seeds. Keywords Chlorophyll · Cold · Hydrogen peroxide · Jasmonic acid · Proline · Soluble sugars · TBARS Abbreviations ABA Abscisic acid BAP Benzylaminopurine Chl Chlorophyll JA Jasmonic acid MDA Malondialdehyde Communicated by Maurizio Lambardi. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11240-020-01924-0) contains supplementary material, which is available to authorized users. * Alina Trejgell [email protected] 1
Department of Plant Biochemistry, Faculty of Biology, University of Warsaw, Warszawa, Poland
Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Toruń, Poland
2
NAA Naphthalene acetic acid ROS Reactive oxygen species TBARS Thiobarbituric acid reactive substances
Introduction Taraxacum pieninicum belongs to the Asteraceae family and is a stenochoric species, occurring in only one site in the Central Pieniny in the Western Carpathians (Poland) (Piękoś-Mirkowa and Mirek 2009). This species is considered to be probably the oldest endemic species in Pieniny Mts. from the Paleogene (Puchalski et al. 2014). Creating collections in botanical garden as a method of its protection is not very effective due to the diffi
Data Loading...
