Molecular characterization of a RING E3 ligase SbHCI1 in sorghum under heat and abscisic acid stress
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ORIGINAL ARTICLE
Molecular characterization of a RING E3 ligase SbHCI1 in sorghum under heat and abscisic acid stress Sung Don Lim1 · Dae Gyeom Oh1 · Yong Chan Park1 · Cheol Seong Jang1 Received: 13 June 2020 / Accepted: 12 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Main conclusion Molecular function ofRING E3 ligase SbHCI1is involved in ABA-mediated basal heat stress tolerancein sorghum. Abstract Global warming generally reduces plant survival, owing to the negative effects of high temperatures on plant development. However, little is known about the role of Really Interesting New Gene (RING) E3 ligase in the heat stress responses of plants. As such, the aim of the present study was to characterize the molecular functions of the Sorghum bicolor ortholog of the Oryza sativa gene for Heat- and Cold-Induced RING finger protein 1 (SbHCI1). Subcellular localization revealed that SbHCI1 was mainly associated with the cytosol and that it moved to the Golgi apparatus under heat stress conditions. The fluorescent signals of SbHCI1 substrate proteins were observed to migrate to the cytoplasm under heat stress conditions. Bimolecular fluorescence complementation (BiFC) and yeast two-hybrid (Y2H) assays revealed that SbHCI1 physically interacted with OsHCI1 ortholog partner proteins in the cytoplasm. Moreover, an in vitro ubiquitination assay revealed that SbHCI1 polyubiquitinated each of the three interacting proteins. The ectopic overexpression of SbHCI1 in Arabidopsis revealed that the protein was capable of inducing abscisic acid (ABA)-hypersensitivity and basal heat stress tolerance. Therefore, SbHCI1 possesses E3 ligase activity and may function as a positive regulator of heat stress responses through the modulation of interacting proteins. Keywords ABA sensitivity · Golgi · Heat stress · Ortholog gene · Rice · RING finger protein · Sorghum
Communicated by Dorothea Bartels. Sung Don Lim and Dae Gyeom Oh contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03469-0) contains supplementary material, which is available to authorized users. * Cheol Seong Jang [email protected] Sung Don Lim [email protected] Dae Gyeom Oh dae‑[email protected] Yong Chan Park [email protected] 1
Plant Genomics Lab, Department of Bio‑Resources Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
Abbreviations RING Really interesting new gene Ub Ubiquitin Y2H Yeast two-hybrid BiFC Bimolecular fluorescence
Introduction Global food production is expected to increase by 70% to meet the needs of the growing human population by 2050 (Tilman et al. 2011). However, extreme heat events related to global warming have already reduced global crop yields by 9–10% from 1964 to 2007 (Lesk et al. 2016). In general, heat stress in plants typically occurs when there is a temperature increase of 10–15 °C above the ambient temperature, and each degree-Celsius increase in mean glob
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