Acute phase protein expressions in secretory and cistern lining epithelium tissues of the dairy cattle mammary gland dur
- PDF / 742,691 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 43 Downloads / 150 Views
RESEARCH ARTICLE
Open Access
Acute phase protein expressions in secretory and cistern lining epithelium tissues of the dairy cattle mammary gland during chronic mastitis caused by staphylococci M. Zalewska1, E. Kawecka-Grochocka2,3, D. Słoniewska3, E. Kościuczuk3,4, S. Marczak5, W. Jarmuż5, L. Zwierzchowski6 and E. Bagnicka3*
Abstract Background: Mastitis is the most common disease in dairy cattle and the costliest for the dairy farming industry, as it lowers milk yield and quality. Mastitis occurs as a result of interactions between microorganisms and the individual genetic predispositions of each animal. Thus, it is important to fully understand the mechanisms underlying these interactions. Elucidating the immune response mechanisms can determine which genetic background makes an animal highly resistant to mastitis. We analyzed the innate immune responses of dairy cows naturally infected with coagulase-positive staphylococci (CoPS; N = 8) or coagulase-negative staphylococci (CoNS; N = 7), causing persistent mastitis (after several failed treatments) vs. infection-free (i.e., healthy [H]; N = 8) dairy cows. The expressions of the acute phase protein genes serum amyloid A3 (SAA3), haptoglobin (HP), ceruloplasmin (CP) genes in the tissues most exposed to pathogens— mammary gland cistern lining epithelial cells (CLECs) and mammary epithelial cells (MECs)—were analyzed. Results: We found constitutive and extrahepatic expressions of the studied genes in both tissue types. HP expression in the MECs of the CoPS-infected group was higher than in the H group (p ≤ 0.05). Moreover, higher SAA3 expression in the CoPS and CoNS groups than in the H group (p = 0.06 and 0.08, respectively) was found. No differences between SAA3 and HP in CLECs were revealed, regardless of the pathogen type. However, higher expression of CP (p ≤ 0.05) in the CoPS group than in the H group was noted. Conclusions: The expressions of selected acute phase proteins were similar between CLECs and MECs, which means that CLECs are not only a mechanical barrier but are also responsible for the biological immune response. Our findings agree with the results of other authors describing the immunological response of MECs during chronic mastitis, but the results for CLECs are novel. Keywords: Dairy cow, Mammary gland, Epithelial cells, Cisternal lining epithelial cells, Chronic mastitis
* Correspondence: [email protected] 3 Department of Biotechnology and Nutrigenomics, Institute of Genetics and Animal Biotechnology PAS, Postępu 36A St., Jastrzębiec, Poland Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this ar
Data Loading...
