Adipose-derived mesenchymal stem cell seeded Atelocollagen scaffolds for cardiac tissue engineering
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TISSUE ENGINEERING CONSTRUCTS AND CELL SUBSTRATES Original Research
Adipose-derived mesenchymal stem cell seeded Atelocollagen scaffolds for cardiac tissue engineering Qiong Li1 Miaomiao Li2 Meng Li1 Zhengyan Zhang3 Han Ma1 Liang Zhao4 Min Zhang5 Guodong Wang ●
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Received: 22 January 2020 / Accepted: 7 September 2020 © The Author(s) 2020
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Abstract ADMSCs were isolated from subcutaneous adipose tissue, characterized and cultured in vitro. GFP-labeled ADMSCs can grow and proliferate well on the Atelocollagen scaffolds, and induced by 5-aza the cells can differentiate into cardio-like cells. 3D cultured ADMSCs on Atelocollagen scaffolds were transplanted into mice ischemia myocardium, and have good biocompatibility with host cardio tissue. Graphical Abstract
1 Introduction Over the past decades, a variety of stem cells have been used in clinical trials. And again, stem cell transplantation, has been a new method for the treatment of myocardial infarction (MI), a
severe clinical condition. In recent years, three types of biological materials have mainly been used for myocardial tissue engineering research: natural materials, synthetic materials, and composite materials. Natural materials mainly include collagen, coral, amino dextran, chondroitin sulfate, gelatin, sodium alginate, fibrin gel, etc. [1–3]. Synthetic materials refer to polyurethane, polyglycolic acid (PGA), polylactic acid (PLA),
* Min Zhang [email protected]
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* Guodong Wang [email protected]
Third Affiliated Hospital, Xinxiang Medical University, Xinxiang 453003, China
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School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China
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The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450008, China
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Nursing School, Xinxiang Medical University, Xinxiang 453003, China
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Henan Medical Tissue Regeneration Key Laboratory, Xinxiang Medical University, Xinxiang 453003, China
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Journal of Materials Science: Materials in Medicine (2020)31:83
polyhydroxybutyrate, etc. [4–6]. Composite materials represent chitosan, polylactic acid and multi-walled carbon nanotube fibers, etc. [7]. Ideal myocardial tissue engineering scaffolds materials should be characterized by: non-toxic and good biocompatibility; easy to form 3D structure; high porosity; good surface activity for cell adsorption; biodegradability and plasticity. Atelocollagen is presently found to be equipped with the abovementioned characteristics and can be used as a tissue filler and for intervertebral disk regeneration culture scaffolds [8]. According to our previous research, Atelocollagen scaffolds could be perfectly combined with neonatal and adult rat cardiomyocytes. In addition, pulsatile 3D myocardial tissue blocks were cultured [9]. Compared to mesenchymal stem cells (MSCs) from other sources, adipose tissue-derived mesenchymal stem cells (ADMSCs) can be isolated easily [10]. ADMSCs have a wide variety of applications, including tissue engineering, regenerative medicine and cell
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