Aerosol microbial community structure analysis based on two different sequencing platforms
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ORIGINAL PAPER
Aerosol microbial community structure analysis based on two different sequencing platforms Xiu-lu Lang . Ai-ling Xu . Xiang Chen . Zhi-wen Song
Received: 6 December 2019 / Accepted: 29 August 2020 Ó Springer Nature B.V. 2020
Abstract As the main biological components of the air ecosystem, airborne microorganisms play an important role in ecosystem function, and most existing studies focus on their health and environmental effects, while ignoring ecological effects. In this study, air microbial samples from two sampling points in the coastal city of Qingdao were collected by a large volume air sampler. The Illumina MiSeq and PacBio high-throughput sequencing methods were used to analyze the microbial community structure and diversity of the two sampling points. The results showed that there were significant differences in the number and relative abundances of microorganisms identified by the two platforms, and with the refinement of the sample classification, the differences in the community became increasingly significant. Proteobacteria and Firmicutes were the dominant bacterial phyla among the airborne microorganisms. MiSeq sequencing showed that the relative abundances of unidentified bacteria were as high as 8.03–16.42% in MiSeq sequencing, while they were almost undetected in PacBio sequencing. Based on the different sequencing platforms, Bacilli, Gammaproteobacteria and Alphaproteobacteria could be seen as significant gaps in the two sampling points. Betaproteobacteria
X. Lang A. Xu X. Chen Z. Song (&) School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China e-mail: [email protected]
showed differences only in the coastal area, while the other 14 classes were differentially abundant in the urban area. Pseudomonas was the main genus in the aerosol, followed by Lactococcus. The relative abundances of bacterial taxa displayed statistically significant differences in the different functional areas. There were more diverse microbes in the urban area than in the coastal area; moreover, the relative abundances of dominant genera showed significant differences, such as Variovorax and Deinococcus. Keywords Microbial community structure Aerosol Illumina MiSeq sequencing PacBio sequencing
1 Introduction Atmospheric particulate matter is ubiquitous in the daily environment; it not only reduces visibility but also raises deep-seated problems such as human survival and environmental ecological crisis. The effects of atmospheric particles on human health, the environment and the ecosystem depend mainly on their source, shape, diameter, chemical composition and attached microorganisms. Among them, the attached microorganisms are the most important part of atmospheric particulate matter, namely bioaerosols. Bioaerosols vary greatly in size from 0.02 to 100 lm
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Aerobiologia
and the respirable fractions (\ 3.3 lm) are particularly important in the epidemiology of diseases (Bragoszewska and Bie
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