Alteration of floral organ identity by over-expression of IbMADS3 - 1 in tobacco
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ORIGINAL PAPER
Alteration of floral organ identity by over-expression of IbMADS3-1 in tobacco Mi-Rae Shin • Sang-Gyu Seo • Ji-Seoung Kim • Seo-Bum Joen • Seung-Won Kang • Gung-Pyo Lee Suk-Yoon Kwon • Sun-Hyung Kim
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Received: 31 January 2010 / Accepted: 8 June 2010 / Published online: 22 June 2010 Ó Springer Science+Business Media B.V. 2010
Abstract The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are important in the development of whole plants including roots, stems, leaves, and the plant vascular system. IbMADS3-1, which is in the STMADS subfamily, and which has been cloned in Ipomoea batatas (L.) Lam., is expressed in all vegetative tissues of the plant, particularly in white fibrous roots. Sequence similarity, besides the spatial and
Mi-Rae Shin and Sang-Gyu Seo should be regarded co-first authors. M.-R. Shin S.-G. Seo J.-S. Kim S.-B. Joen S.-H. Kim (&) Department of Environmental Horticulture, University of Seoul, Seoul 130-743, Korea e-mail: [email protected] S.-W. Kang G.-P. Lee Department of Applied Plant Science, College of Industrial Science, Chung-ang University, Anseing-si, Gyeinggi-do 456-756, Korea
temporal expression patterns, enabled the definition of a novel MADS-box subfamily comprising STMADS16 and the other MADS-box genes in STMADS subfamily expressed specifically in vegetative tissues. Expression of IbMADS3-1 was manifest by the appearance of chlorophyll-containing petals and production of characteristic changes in organ identity carpel structure alterations and sepaloidy of the petals. In reverse transcription-polymerase chain reaction analysis with a number of genes known to be key regulators of floral organ development, the flowering promoter NFL1 was clearly reduced at the RNA level compared with wild type in transgenic line backgrounds. Moreover, NtMADS5 showed slight down-regulation compared with wild-type plants in transgenic lines. These results suggest that IbMADS3-1 could be a repressor of NFL1 located upstream of NtMADS5. IbMADS3-1 ectopic expression is suggested as a possible means during vegetative development by which the IbMADS3-1 gene may interfere with the floral developmental pathway. Keywords IbMADS3-1 MADS-box gene STMADS subfamily Sweet potato (Ipomoea batatas L. Lam) Vegetative development
Introduction S.-Y. Kwon Systemic Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Taejon 305-806, Korea
During the last decade, a huge amount of genetic and molecular information about Arabidopsis, Antirrhinum,
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and rice has accumulated. These collective research efforts have led to increased understanding of the complexity of development in higher plants (Aswath and Kim 2005). The initiation of organized development is a complex mo
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