Overexpression of GmGolS2-1 , a soybean galactinol synthase gene, enhances transgenic tobacco drought tolerance
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ORIGINAL ARTICLE
Overexpression of GmGolS2-1, a soybean galactinol synthase gene, enhances transgenic tobacco drought tolerance Shuang Qiu1 · Jun Zhang2 · Jiaqi He1 · Wei Sha1 · Mingyang Li1 · Yan Zhao1 · Ying Zhai1 Received: 6 July 2020 / Accepted: 14 September 2020 © Springer Nature B.V. 2020
Abstract Plant adaptation to abiotic stress is significantly influenced by galactinol synthase (GolS), which is a regulatory enzyme that catalyzes the synthesis of raffinose family oligosaccharides (RFOs). Soybean GolS genes, GmGolS2-1 and GmGolS2-2 were isolated in this study. GmGolS2-1 was 993 bp and encoded a 330-amino acid polypeptide, and GmGolS2-2 was 975 bp and encoded a 324-amino acid polypeptide. The deduced amino acid sequences contained common features of plant GolS proteins: a DXD motif and a hydrophobic pentapeptide (APSAA). Significant increase of GmGolS2-1 and GmGolS2-2 transcripts under drought stress was observed under real-time fluorescence quantitative PCR (qPCR), while their transcripts only increased slightly under salt and cold stresses. The GmGolS2-1 promoter region contained four methyl jasmonate-responsive elements, two anaerobic-induced elements, a wound-responsive element, and two MYB binding sites. Overexpression of GmGolS2-1 enhanced the GolS activity along with expression of stress-associated genes in transgenic tobacco. Additionally, the overexpression of GmGolS2-1 resulted in lower relative electrolyte leakage and malondialdehyde (MDA) content and higher soluble carbohydrates and proline content in transgenic tobacco than wild-type (WT) tobacco under drought stress. These findings indicate that GmGolS2-1 improved transgenic tobacco drought resistance. Key message Two galactinolsynthase genes, GmGolS2-1 andGmGolS2-1, were isolated from soybean. They could be inducedby abiotic stress. GmGolS2-1 enhancestolerance to drought in transgenic tobacco. Keywords GmGolS2-1 · Soybean · Galactinol synthase · Overexpression · Drought tolerance Abbreviations GolS Galactinol synthase MDA Malondialdehyde ORF Open reading frame PCR Polymerase chain reaction PEG Polyethylene glycol qPCR Quantitative real-time PCR RFOs Raffinose family oligosaccharides
Communicated by: Francisco de Assis Alves Mourão Filho. * Ying Zhai [email protected] 1
College of Life Science and Agroforestry, Qiqihaer University, Qiqihaer 101006, Heilongjiang, China
Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar 161005, Heilongjiang, China
2
ROS Reactive oxygen species WT Wild-type
Introduction The yield, development and growth of plants can be seriously impaired by abiotic stress, including low or high temperatures, drought, and salinity. Crops have developed complex strategies to increase osmotic pressure and to enhance their stress resistance to reduce the possible damage from this stress. These include small soluble molecule accumulation. Typical representatives of these small soluble molecules are raffinose family oligosaccharides (RFOs), which act as osmop
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