Altered brassinolide sensitivity1 transcriptionally inhibits chlorophyll synthesis and photosynthesis capacity in tomato
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ORIGINAL PAPER
Altered brassinolide sensitivity1 transcriptionally inhibits chlorophyll synthesis and photosynthesis capacity in tomato Muhammad Ali Mumtaz1 · Shoaib Munir1 · Genzhong Liu1 · Weifang Chen1 · Ying Wang1 · Huiyang Yu1 · Saira Mahmood1 · John Kojo Ahiakpa1 · Safir Ahmad Tamim1 · Yuyang Zhang1 Received: 9 May 2020 / Accepted: 7 August 2020 © Springer Nature B.V. 2020
Abstract Brassinolide (BR) enhances the efficiency of photosynthetic machinery through the activation of metabolic pathways, photochemistry, and ribulose activity. However, the potential transcriptional mechanism mediating these processes through BR signaling still needed to be elucidated. In this study, we found the concealed BR-signaling mediated effects on tomato leaf morpho-physiological and biochemical traits, including chlorophyll accumulation, and photosynthetic efficiency. Under controlled conditions, the altered brassinolide sensitivity1 (abs1) mutant showed decreased leaf area and biomass associated with substantially reduced vascularization and epidermal cell size. abs1 mutant displayed significantly decreased chlorophyll accumulation and suppression in photosynthetic machinery components like photochemical quenching, electron transport rate, the maximal quantum yield of PSII photochemistry, and net photosynthetic rate. Whereas an increase in minimal fluorescence yield and non-photochemical quenching, suggests that abs1 mutant leaf has weakened abilities to harvest and transfer light energy. Moreover, the transcriptome analysis revealed differentially expressed genes involved in the chlorophyll biosynthesis and photosystem (PSI and PSII) reaction center. The abs1 mutant depicted the decreased expression level of genes encoding light-harvesting chlorophyll a/b binding proteins and photosystem II binding protein A required for the reaction center of the PSII complex. In addition, hormonal profiling of the abs1 mutant indicates the complexity of the BR and other phytohormones interactions. Our findings concluded that the BR signaling reduction transcriptionally impairs chlorophyll synthesis, quantum photon harvesting, and light energy transfer, leading to a decrease in photosynthetic capacity. Keywords BR insensitivity · Chlorophyll accumulation · Chlorophyll fluorescence · Photosynthesis · Tomato
Introduction Photosynthesis is a universal biochemical process, through which chlorophyll converts solar energy into biomass. It covers the basic oxygen demand and plays a vital role in feeding the hungry biota of the earth (Voitsekhovskaja and Tyutereva 2015). Chlorophyll is an essential component of photosynthesis, which plays a crucial role in photosynthetic reactions (Ahammed et al. 2012) for harvesting and transportation of Electronic supplementary material The online version of this article (doi:https://doi.org/10.1007/s10725-020-00650-z) contains supplementary material, which is available to authorized users. * Yuyang Zhang [email protected] 1
Key Laboratory of Horticultural Plant Biology, Ministry of Education,
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