Analysis of a cellulose synthase catalytic subunit from the oomycete pathogen of crops Phytophthora capsici
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ORIGINAL RESEARCH
Analysis of a cellulose synthase catalytic subunit from the oomycete pathogen of crops Phytophthora capsici Zhili Pang . Lauren S. McKee . Vaibhav Srivastava . Stefan Klinter . Sara M. Dı´az-Moreno . Peter Orlean . Xili Liu . Vincent Bulone
Received: 11 March 2020 / Accepted: 20 July 2020 Ó The Author(s) 2020
Abstract Phytophthora capsici Leonian is an important oomycete pathogen of crop vegetables, causing significant economic losses each year. Its cell wall, rich in cellulose, is vital for cellular integrity and for interactions with the host organisms. Predicted cellulose synthase (CesA) proteins are expected to catalyze the polymerization of cellulose, but this has not been biochemically demonstrated in an oomycete. Here, we present the properties of the four newly identified CesA proteins from P. capsici and compare Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10570-020-03362-9) contains supplementary material, which is available to authorized users. Z. Pang L. S. McKee V. Srivastava S. Klinter S. M. Dı´az-Moreno V. Bulone (&) Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden e-mail: [email protected]
their domain organization with that of CesAs from other lineages. Using a newly constructed glucosyltransferase-deficient variant of Saccharomyces cerevisiae with low residual background activity, we have achieved successful heterologous expression and biochemical characterization of a CesA protein from P. capsici (PcCesA1). Our results demonstrate that the individual PcCesA1 enzyme produces cellobiose as the major reaction product. Co-immunoprecipitation studies and activity assays revealed that several PcCesA proteins interact together to form a complex whose multiproteic nature is most likely required for cellulose microfibril formation. In addition to providing important insights into cellulose synthesis in the P. Orlean Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA V. Bulone ARC Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia
Z. Pang X. Liu (&) Department of Plant Pathology, China Agricultural University, Beijing 100193, People’s Republic of China e-mail: [email protected] L. S. McKee Wallenberg Wood Science Centre, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden
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Cellulose
oomycetes, our data may assist the longer term identification of cell wall biosynthesis inhibitors to control infection by pathogenic oomycetes. Keywords Cellulose synthase Oomycete Crop pathogen Phytophthora capsici Cell wall biosynthesis
Introduction Phytophthora capsici Leonian (Leonian 1922) has a devastating epidemiology (Hausbeck and Lamou
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