Overexpression of the bamboo sucrose synthase gene ( BeSUS5 ) improves cellulose production, cell wall thickness and fib
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ORIGINAL ARTICLE
Overexpression of the bamboo sucrose synthase gene (BeSUS5) improves cellulose production, cell wall thickness and fiber quality in transgenic poplar Yan Huang 1
&
Lili Wang 1 & Shanglian Hu 1 & Xuegang Luo 1 & Ying Cao 1
Received: 13 March 2020 / Revised: 25 July 2020 / Accepted: 25 August 2020 / Published online: 15 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract In plants, sucrose synthase (SUS, EC 2.4.1.13) is widely considered a multifunctional protein involved in modulating sink strength, cellulose biosynthesis, and carbon partitioning. However, supporting genetic evidence regarding the role of SUS from bamboo in fiber development is lacking. Here, we obtained transgenic poplar lines overexpressing the bamboo BeSUS5 gene and conducted functional analysis. We found that overexpression of BeSUS5 enhanced the activity of SUS and significantly promoted the growth of the plants, especially xylem growth. In BeSUS5 overexpressed poplar plants, the total soluble sugar (TSS) and starch contents were decreased in leaves, while the cellulose content was increased in stems, indicating that overexpression of BeSUS5 might enhance the partitioning of carbon to cellulose in poplar. Consistent with these results, the expression of cellulose biosynthesis and phloem loading–related genes, such as cellulose synthase (CesA7), KORRIGAN (KOR), and sucrose transporter (SUT1), was upregulated in transgenic plants. As a result, transgenic poplars displayed not only an increase in cell wall thickness and cell wall crystallinity but also an altered stem fiber phenotype. Taken together, our results imply the vast potential of BeSUS5 for the genetic improvement of wood cellulose production and fiber quality. Keywords Bamboo . Poplar . Sucrose synthase . Cellulose biosynthesis . Fiber phenotype . Carbon partitioning
Introduction The plant cell wall is mainly composed of cellulose, hemicellulose, and lignin, which not only provide mechanical support for the plant and aid in avoiding biotic and abiotic stresses but also provide plentiful raw materials (Cassab 1998; Somerville 2006; Kumar et al. 2016). Any changes in the composition or structural properties of cell walls may have significant effects Yan Huang and Lili Wang contributed equally to this work. Communicated by A. De La Torre Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11295-020-01464-w) contains supplementary material, which is available to authorized users. * Shanglian Hu [email protected] * Xuegang Luo [email protected] 1
School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
on plant morphology, growth potential, and biomass (Niu et al. 2018). Among these compounds, cellulose is the major carbon reservoir in plants, and its content and availability can impact the conversion and production of biofuel as well as affect the yield of pulp and the quality of paper (Jin et al. 2017). Cellulose is a type of poly
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