Ancient DNA Typing Methods, Strategies and Applications
Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environment
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Susanne Hummel· Ancient DNA Typing Methods, Strategies and Applications
Springer-Verlag Berlin Heidelberg GmbH
Susanne Hummel
Ancient DNA Typing Methods, Strategies and Applications
With 152 Figures and 34 Tables
Springer
Dr.
SUSANNE HUMMEL
Historische Anthropologie und Humanokologie Institut fUr Zoologie und Anthropologie Georg August Universitat Gottingen Biirgerstra6e 50 37073 Gottingen Germany
Library of Congress Cataloging. in-Publication Data Hummel, Susanne Ancient DNA typing: methods, strategies, and applications 1 Susanne Humel. p.cm. Includes bibliographical references and index. \. DNA, Fossil--Analysis. 2. Mitochondrial DNA--Analysis. 3. Nucleotide sequence. 4. Polymerase chain reaction.!. Title. This work is subject to copyright. All rights reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9,1965, in its current version, and permission for use must always be obtained from Springer-Veriag. Violations are liable for prosecution under the German Copyright Law. http://www.springer.de ISBN 978-3-642-07705-0 ISBN 978-3-662-05050-7 (eBook) DOl 10.1007/978-3-662-05050-7 © Springer-Verlag Berlin Heidelberg 2003 Originally published by Springer-Verlag Berlin Heidelberg New York in 2003. Solleover reprint of the hardcover 1st edition 2003
The use of general descriptive names, registered names, trademarks~ etc. in this publication does not imply, even in
the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: Design & Production, Heidelberg Typesetting: Cameraready by the author SPIN 10645234 31/3150 - 5432 10- Printed on acid-free paper
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Preface
Who does not remember the genetic identification of the skeletons of the Romanov family or the investigation of the putative clothing of the famous Kaspar Hauser? Those are highlights in the analysis of ancient DNA, a field that has come a long way in the last decade. The topic of ancient DNA analysis and the possible future implications inspire both the public and the scientific community. This book addresses scientific practitioners and graduate students from different disciplines interested in ancient and degraded DNA research. The book is divided into chapters about methods and strategies, protocols and applications, and serves both as a laboratory manual and as a readable textbook in a meanwhile highly specialized field. It was possible to write this book because of the experience accumulated in ancient DNA typing and the committed efforts of many people who worked in the G6ttingen laboratory for ancient DNA research over the last decade. It was mainly these colleagues who carried out experiments
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