Animal hydatid cyst genotypes as a potential substitute for human hydatid cyst as a source of antigen for diagnosis of z
- PDF / 797,461 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 57 Downloads / 203 Views
ORIGINAL ARTICLE
Animal hydatid cyst genotypes as a potential substitute for human hydatid cyst as a source of antigen for diagnosis of zoonotichydatidosis Reem M. Ramadan1 • Marwa M. Khalifa1 • Dina M. El-Akkad2 • Azza M. Abdel-Wahab1 • Mohamed M. El-Bahy1
Received: 3 October 2020 / Accepted: 2 November 2020 Ó Indian Society for Parasitology 2020
Abstract The Diagnosis of hydatidosis is still an unsolved issue due to difficulties in obtaining of patient’s hydatid cyst appropriate for antigen extraction. This study evaluated the suitability of HC protoscolices somatic antigens (HCPsS-Ag) fractions from animal origin to substitute that extracted from HC of patients in diagnosis of hydatidosis using enzyme-linked immunoelectro-transfer blot and Enzyme-linked immunosorbent assay (ELISA). Eight fractions in HC-G6 from patients react specifically versus HC-G6 infected patient’s sera. Five of them (28, 32, 38, 59 and 89 Kilo Dalton (KDa) and two of them (28 KDa and 45 KDa) reacted versus HC-G1 and HC-G4 infected sheep and equine sera, respectively. Six fractions in HCPsS-AgG1 of sheep react versus HC-G1 sheep infected sera, four (28, 32, 52 and 58 KDa) and two of them reacted versus HC-G6 and HC-G4 infected patient and equine sera, respectively. Two fractions only in HCPsS-Ag-G4 of equine reacted versus infected human and sheep sera. This fraction displayed the same degree of ELISA value versus different infected sera with a significantly perfect classification for kappa agreement and non-statistically significant difference (p C 0.05) for ELISA Optical density values of the positive samples without cross-reaction versus other parasites antibodies in sera. HCPsS-Ag from HC genotypes that developed in humans and animals as HC-G6 and HCG1 can substitute each other for diagnosis of infection than antigens extracted from non-zoonotic HC-G4. The fraction & Mohamed M. El-Bahy [email protected]; [email protected] 1
Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt
2
Department of Parasitology, Faculty of Medicine, Cairo University, Giza, Egypt
at 28 KDa is the only fraction that can be extracted from any animals HC and used in diagnosis of zoonotic hydatidosis. Keywords Antigen fraction EITB ELISA Hydatidosis Human and animal protoscolices
Introduction Hydatidosis or cystic echinococcosis is a silent cyclozoonotic disease-causing public health problem among humans and animals in underdeveloped countries all over the world. The disease is caused by the larvae HC of Echinococcus species. This tapeworm developed in the small intestine of dogs as the final host and is transmitted in a synanthropic cycle between dogs, humans, and livestock (Siracusano et al. 2012). Cystic echinococcosis infection is mostly asymptomatic, with variable and non-specific symptoms. Recently, imaging methods associated with immunological methods are described as a tool for confirming clinical symptoms for the diagnosis in patients (Fotoohi et al. 2013). Due to difficulty in obtaining fresh HC
Data Loading...
