Anti-Diabetes Components in Leaves of Yacon
The inhibitory effect of four new compounds (smallanthaditerpenic acids A–D) previously isolated from leaves of Smallanthus sonchifolius (yacon) on α-glucosidase were examined and their IC50 were determined to be 0.48, 0.59, 1.00, and 1.17 mg/mL respectiv
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Anti-Diabetes Components in Leaves of Yacon Zheng Xiang, Chen-Feng Ji, De-Qiang Dou and Kuo Gai
Abstract The inhibitory effect of four new compounds (smallanthaditerpenic acids A–D) previously isolated from leaves of Smallanthus sonchifolius (yacon) on a-glucosidase were examined and their IC50 were determined to be 0.48, 0.59, 1.00, and 1.17 mg/mL respectively. In addition, a rapid, reliable HPLC method for the analysis of smallanthaditerpenic acids A and C in yacon leaves was established, and the variation in their contents in leaves from plants cultivated in different places and collected at different times of the year were compared. The established analytical method for determining smallanthaditerpenic acids A and C presented good results and could be used as a method for the quality control of S. sonchifolius leaves. Keywords Smallanthus sonchifolius HPLC
Smallanthaditerpenic acid
Diabetes
434.1 Introduction Yacon, Smallanthus sonchifolius (Peoepp. & Endl.) H. Robinson (family Asteraceae), an indigenous plant of the Andes, was introduced into China via Japan in the early 1990s. It has been reported that its leaf extract has potential Z. Xiang C.-F. Ji D.-Q. Dou (&) Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin University of Commerce, Harbin 150076, China e-mail: [email protected] Z. Xiang C.-F. Ji D.-Q. Dou Center of Research on Life Science and Environmental Science, Harbin University of Commerce, Harbin 150076, China D.-Q. Dou K. Gai College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
S. Li et al. (eds.), Frontier and Future Development of Information Technology 3353 in Medicine and Education, Lecture Notes in Electrical Engineering 269, DOI: 10.1007/978-94-007-7618-0_434, Springer Science+Business Media Dordrecht 2014
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antidiabetic effects [1]. Chemical investigations of yacon have revealed that its leaves contain monoterpenes, sesquiterpenes and diterpenes, which have a physiological role in the pest-resistant and antimicrobial activities of the plant [2–4]. Previously, kaurene derivatives, pentacosaol, cannabiside D, platanionoside B, octadecatrienoic acid and smallanthaditerpenic acids A, B, C and D (Fig. 434.1) have been isolated from leaves of yacon cultivated in China [5–7]. In continuation of this work, we found that smallanthaditerpenic acids (SA) A, B, C and D possessed stronger inhibitory activity of a-glucosidase than that of caffeic acid, which had been regarded as the major component possessing this property [8]. An HPLC method has also been developed and validated for the quantification of SAs A and C in yacon and used to study the variation of these compounds in leaves collected from plants cultivated at different places and collected at different times of the year.
434.2 Results and Discussion The inhibitory effect of SA A*D on a-glucosidase was determined, which indicated that they possess strong inhibitory activity (Table 434.1). In this experimen
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