Effects of Temperature on Transcriptome Profiles in Cardiocrinum cathayanum Leaves
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Effects of Temperature on Transcriptome Profiles in Cardiocrinum cathayanum Leaves X. F. Wanga, b, *, Y. J. Yeb, M. Y. Fanb, L. Chenb, T. Mab, and Z. B. Wanb aHuangshan
Vocational and Technical College, Huangshan City, Anhui Province, 245000 P. R. China University, Huangshan City, Anhui Province, 245021 P. R. China *e-mail: [email protected]
bHuangshan
Received August 24, 2019; revised April 29, 2020; accepted April 29, 2020
Abstract—Cardiocrinum cathayanum (Endl.) Lindl. (Liliaceae) is a promising species for ornamental and pharmaceutical usage. However, genomic responses of C. cathayanum to temperature have not been investigated. In the present study, C. cathayanum leaves were cultivated at 10, 20, and 30°C, and leaves were collected for transcriptome sequencing. Overall, 36.9 to 44.1 M clean reads were obtained, which finally assembled 48120 unigenes. Among these unigenes, 49.39% could be annotated to at least one public database. Compared with 20°C treatment, 381 and 303 unigenes were significantly upregulated, 580 and 1399 were significantly downregulated in 10 and 30°C treatments, respectively. Real-time qPCR analyses on selected 12 unigenes showed similar tendencies to those calculated on transcriptome sequencing. Compared with 20°C treatment, exposure to 10°C significantly affected the pentose and glucuronate interconversions, starch and sucrose metabolism pathways. Further exploration suggested that D-galacturonate and trehalose were accumulated at 10°C, which would enhance the tolerance to cold stress. Besides, 10°C treatment inhibited the biosynthesis of unsaturated fatty acids and α-linolenic acid metabolism. These changes might negatively affect C. cathayanum growth. At 30°C, 40 of 42 differentially expressed genes (DEGs) in the starch and sucrose metabolism, all the 27 DEGs in the glycolysis/gluconeogenesis and all the 17 DEGs in the fructose and mannose metabolism were significantly downregulated, in comparison to 20°C treatment. These changes demonstrated that high temperature greatly inhibited carbohydrate metabolism in C. cathayanum leaves. Overall, the present study contributed new insights to understand the genomic responses of C. cathayanum leaves to various temperatures. Keywords: Cardiocrinum cathayanum, differentially expressed gene, growth, Gene Ontology, KEGG pathway, hot stress, cold stress DOI: 10.1134/S1021443720060199
INTRODUCTION Lilies are flowers commercially available worldwide. Lily is the common name of plants belonging to Liliaceae: Lilium. Due to their beauty and pharmaceutical values, lilies are popular as ornamental plants and fresh cut-flowers. In addition to Lilium, another genus in Liliaceae, Cardiocrinum, has similar or even higher ornamental value. Plants of Cardiocrinum are larger than those of Lilium, with shoot length ranging from 100 to 150 cm. Thus, they have the common name giant lily. Moreover, Cardiocrinum spp. have large, attractive flowers and high contents of bioactive comAbbreviation: COG/KOG—cluster of orthologous groups of proteins database
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