Antibacterial Activity and Mechanism of Action of 10-HDA Against Escherichia coli
10-HDA (10-hydroxy-2-decenoic acid), an unsaturated fatty acid, is the main active component of royal jelly. It has been demonstrated that 10-HDA possesses remarkable antibacterial activities. However, it is unclear in terms of the underlying antibacteria
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Antibacterial Activity and Mechanism of Action of 10-HDA Against Escherichia coli Xiaohui Yang, Tengfei Wang and Ruiming Wang
Abstract 10-HDA (10-hydroxy-2-decenoic acid), an unsaturated fatty acid, is the main active component of royal jelly. It has been demonstrated that 10-HDA possesses remarkable antibacterial activities. However, it is unclear in terms of the underlying antibacterial mechanism of 10-HDA. In our study, we showed that 10HDA has obvious effects against Gram-negative bacteria and its antibacterial activity was not affected by pH and temperature. 10-HDA was shown to change membrane ionic conductivity and resulted in the leakage of K+, Ca2+, and Mg2+ from bacterial cells, suggesting that 10-HDA was able to compromise the integrity of the bacterial membranes. By detecting the 260 nm absorbing materials, it was showed that the membrane integrity was damaged and resulted in the leakage of large molecules. The results suggested that 10-HDA could damage the permeability and integrity of bacterial cell membrane, leading to the outflow of important large molecules from the cell and its eventual death. Keywords 10-HDA permeability
Antibacterial mechanism Escherichia coli Membrane
Xiaohui Yang and Tengfei Wang contributed equally to this article. X. Yang T. Wang R. Wang (&) Key Laboratory of Shandong Microbial Engineering, Qilu University of Technology, Jinan 250353, China e-mail: [email protected] T. Wang Key Laboratory of Industrial Microbiology, Ministry of Education, Qilu University of Technology, Tianjin 300457, China
T.-C. Zhang et al. (eds.), Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), Lecture Notes in Electrical Engineering 249, DOI: 10.1007/978-3-642-37916-1_60, Ó Springer-Verlag Berlin Heidelberg 2014
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60.1 Introduction 10-HDA (10-hydroxy-2-decenoic acid), an unsaturated fatty acid, is the main active component of royal jelly, which has been mainly found in the royal jelly by now. It has been demonstrated that 10-HDA possesses remarkable biologic activities in antibacterial [1, 2], immunoregulation [3], antioxidative [4, 5], collagen production promoting [6, 7], and antitumour activities [8–10]. The presence of antimicrobial activity in RJ secreted from the pharyngeal glands of the honeybee has been documented for more than 60 years [11–13]. The inhibitory activity of RJ against both Gram-positive and Gram-negative bacteria has been demonstrated. Antibacterial activity of ether-soluble fraction of RJ was rather stronger than that of raw RJ (containing 10-HDA). It was point out that the main component of ether-soluble fraction was 10-HDA occupying 38 % of total acids [12, 14]. The antibacterial activity of 10-HDA against Bacillus subtilis, Staphylococcus aureus, Escherichia coli was stronger than that of the other C10 fatty acids [15]. Extracts of royal jelly as well as its isolated compounds were studied for their antimicrobial activity against two Gram-positive bacteria, S.aureus (ATCC 25923) and S. epidermidis (AT
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