Antimicrobial Peptide JH-3 Effectively Kills Salmonella enterica Serovar Typhimurium Strain CVCC541 and Reduces Its Path
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Antimicrobial Peptide JH-3 Effectively Kills Salmonella enterica Serovar Typhimurium Strain CVCC541 and Reduces Its Pathogenicity in Mice Lei Wang 1,2,3 & Xueqin Zhao 1,4 & Xiaojing Xia 1 & Chunling Zhu 1 & Wanhai Qin 5 & Yanzhao Xu 1 & Bolin Hang 1 & Yawei Sun 1 & Shijun Chen 1 & Huihui Zhang 1 & Jinqing Jiang 1 & Jianhe Hu 1,2 & Hanna Fotina 4 & Gaiping Zhang 3
# Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Salmonella is an important zoonotic pathogen and is a major cause of gastrointestinal diseases worldwide. The current serious problem of antibiotic abuse has prompted the search for new substitutes for antibiotics. JH-3 is a small antimicrobial peptide with broad-spectrum bactericidal activity. In this study, we showed that JH-3 has good bactericidal activity towards the clinical isolate Salmonella enterica serovar Typhimurium strain CVCC541. The minimum inhibitory concentration (MIC) of JH-3 against this bacterium was determined to be 100 μg/mL, which could decrease the number of CVCC541 cells by 1000-fold in vitro within 5 h. The transmission electron microscopy (TEM) results showed that JH-3 can damage the cell wall and membrane of CVCC541, leading to the leakage of cell contents and subsequent cell death. To measure the bactericidal activity of CVCC541-infected mice were treated intraperitoneally 40 or 10 mg/kg JH-3 at 2 h or 3 days postinfection. Our results showed that treatment with 40 mg/kg JH-3 at 2 h postinfection had the best therapeutic effect and could significantly protect mice from a lethal dose of CVCC541. Furthermore, the clinical symptoms, bacterial burden in blood and organs, and intestinal pathological changes were all decreased and were close to normal. This study examined the therapeutic effect of the antimicrobial peptide JH3 against S. enterica CVCC541 infection for the first time and determined the therapeutic effect of different JH-3 doses and treatment times, laying the foundation for studies of new antimicrobial agents. Keywords Antimicrobial peptide . Salmonella . Sterilisation . Enteritis . Pathogenicity
Introduction
* Jianhe Hu [email protected] Lei Wang [email protected] 1
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, People’s Republic of China
2
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, People’s Republic of China
3
Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang, People’s Republic of China
4
Sumy National Agrarian University, Sumy, Ukraine
5
Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands
Salmonella is an important zoonotic pathogen that is a major cause of gastrointestinal disease worldwide [1], and approximately 21 million cases of typhoid fever are reported annually worldwide [2]. The genus Salmonella is of particular clinical relevance in developed and developing countries, where this pa
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