Antioxidant activity and calcium bioaccessibility of Moringa oleifera leaf hydrolysate, as a potential calcium supplemen
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Antioxidant activity and calcium bioaccessibility of Moringa oleifera leaf hydrolysate, as a potential calcium supplement in food Ye-Rang Yun1 • Su-Jin Oh1 • Min-Jung Lee1 • Yun-Jung Choi1 • Sung Jin Park1 Mi-Ai Lee1 • Sung-Gi Min1 • Hye-Young Seo2 • Sung-Hee Park1
•
Received: 4 June 2020 / Revised: 20 August 2020 / Accepted: 25 August 2020 Ó The Korean Society of Food Science and Technology 2020
Abstract Moringa oleifera leaf (ML) is rich in vitamins and minerals, specially abundant calcium, therefore it is widely used as a calcium supplement for food. This study aimed to investigate the antioxidant activity and calcium bioaccessibility of M. oleifera leaf hydrolysate (MLH) as a calcium supplement for kimchi. MLH was prepared under three different proteases, two different protease contents, and three different incubation times. Total phenol content (TPC), total flavonoid content (TFC), and antioxidant
& Sung-Hee Park [email protected] Ye-Rang Yun [email protected]
activities were investigated. Cellular activity and calcium bioaccessibility were also investigated. The highest calcium level of MLH was observed in 3% Protamex treatment for 4 h. TPC, TFC, and antioxidant activities of MLH in Protamex and Alcalase treatments were higher than those in Flavourzyme treatment (p \ 0.05). Moreover, high cell viability and alkaline phosphatase activity were also observed in C2C12 cells. Kimchi containing MLH showed high calcium accessibility compared to kimchi alone. Taken together, the application of MLH could have potential as a calcium supplement for kimchi production. Keywords Antioxidant activity Alkaline phosphatase activity Bioaccessibility Calcium Moringa oleifera leaf hydrolysate Protease
Su-Jin Oh [email protected] Min-Jung Lee [email protected]
Introduction
Yun-Jung Choi [email protected]
Moringa oleifera is broadly farmed in many regions of South Asia, including India and Pakistan (Leone et al., 2015). It is commonly called moringa, drumstick tree, and horseradish tree (Fahey, 2005). Recently, Moringa oleifera has become popular as a food supplement due to its high vitamin, mineral, and protein content. Especially, its leaves are the most nutritious part, leading to their application in many fields, including food (Devisetti et al., 2016), pharmacy (Monera-Penduka et al., 2017), and medicine (Chin et al., 2018). Devisetti et al. (2016) reported that moringa leaf flour (MLF)-based snack products exhibit a highly acceptable texture for sensory evaluation and improved nutritional profile. Interestingly, moringa leaf (ML) extract was designed and developed as a dressing film for wound healing purposes in the field of tissue engineering (Chin
Sung Jin Park [email protected] Mi-Ai Lee [email protected] Sung-Gi Min [email protected] Hye-Young Seo [email protected] 1
Industrial Technology Research Group, Research and Development Division, World Institute of Kimchi, Nam-Gu, Gwangju 61755, Republic of Korea
2
Hygienic Safety and Analysis Center, Research and Development Division, World
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