Application of light microscopical and ultrastructural immunohistochemistry in the study of goblet cell carcinoid in the
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Application of light microscopical and ultrastructural immunohistochemistry in the study of goblet cell carcinoid in the appendix Maya V Gulubova*1, Yovcho Yovchev2, Tatyana Vlaykova3, Philip Hadjipetkov2, Diana K Prangova1 and Angel Popharitov2 Address: 1Department of General and Clinical Pathology, Medical Faculty, Trakia University, Stara Zagora, 11 Armeiska Str., Stara Zagora, Bulgaria, 2Department of General Surgery, Medical Faculty, Trakia University, Stara Zagora, Bulgaria and 3Department of Chemistry and Biochemistry, Medical Faculty, Trakia University, Stara Zagora, Bulgaria Email: Maya V Gulubova* - [email protected]; Yovcho Yovchev - [email protected]; Tatyana Vlaykova - [email protected]; Philip Hadjipetkov - [email protected]; Diana K Prangova - [email protected]; Angel Popharitov - [email protected] * Corresponding author
Published: 6 February 2008 World Journal of Surgical Oncology 2008, 6:15
doi:10.1186/1477-7819-6-15
Received: 23 May 2007 Accepted: 6 February 2008
This article is available from: http://www.wjso.com/content/6/1/15 © 2008 Gulubova et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Goblet cell carcinoids appear less frequently in the appendix than do other carcinoids. In the presented work a case with a goblet cell carcinoid of the appendix is described. Methods: Routine histological and histochemical methods were employed, with a combination of histochemistry and immunohistochemistry on one section and light and electron microscopical immunohistochemisty on paraffin-embedded material, were applied to identify the type of the carcinoid and to reveal the fine structure of cell types in the tumour nests of the appendix. Results: During the biopsy of a patient who had undergone appendectomy, an infiltration with clusters of goblet cells in the submucosa of the appendix was found. After a second operation of right-sided hemicolectomy, similar clusters of goblet cells were detected in the muscle layers of the caecum. After 18 months the patient died from cirrhosis and had not developed metastases or any recurrence. Immunohistochemically the serotonin-, somatostatin-, chromogranin A- and synaptophysin-positive endocrine cells were basally attached to mucin-secreting cells. The combined staining revealed simultaneously present endocrine cells (chromogranin-A-positive) and mucin-secreting cells (PAS- or alcian blue-positive). The ultrastructural immunohistochemistry showed that chromogranin A-positive cells had discoid and pleomorphic granules and were located in tumour nests or as single cells in the appendiceal wall. Conclusion: The combined histochemical and immunohistochemical procedure and the ultrastructural immunohistochemistry on archival material could contribu
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