Application of NIR spectroscopy for the quality control of mangosteen pericarp powder: quantitative analysis of alpha-ma
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ORIGINAL PAPER
Application of NIR spectroscopy for the quality control of mangosteen pericarp powder: quantitative analysis of alpha-mangostin in mangosteen pericarp powder and capsule Jomjai Peerapattana • Kuniko Otsuka Makoto Otsuka
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Received: 14 May 2012 / Accepted: 18 July 2012 / Published online: 25 August 2012 The Japanese Society of Pharmacognosy and Springer 2012
Abstract Near-infrared spectroscopy (NIR) was applied to the quantitative analysis of the concentration of alphamangostin (aM) in mangosteen pericarp powder (MP). The predicted results from the partial least squares chemometric method of various pretreatment data were compared to obtain the best calibration model. Two different types of containers (transparent capsules and glass vials) filled with the same samples were measured. For MP mixture in vials, the calibration model involving nine principal components (PC) could predict the amount of aM most accurately based on non-pretreatment spectral data. For MP mixture in capsules, the calibration model involving nine PC could predict the amount of aM most accurately based on first-derivative pretreatment spectra. The relationships of the calibration models for both samples had sufficiently linear plots. The standard error of cross-validation for the MP mixture in vials was lower and the R2 values of validation were higher compared to the MP mixture in capsules. The equation for prediction of the concentration of aM in MP mixtures
J. Peerapattana Faculty of Pharmaceutical Sciences, Center for Research and Development of Herbal Health Products, Khon Kaen University, Khon Kaen 40002, Thailand K. Otsuka Yokohama College of Pharmacy, Matano-cho 601, Totsuka, Yokohama 245-0066, Japan M. Otsuka (&) Faculty of Pharmacy, Research Institute of Pharmaceutical Sciences, Musashino University, Shinmachi 1-1-20, Nishi-Tokyo 202-8585, Japan e-mail: [email protected]
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in vials is y = 0.9775x ? 0.0425 with R2 = 0.9950 and for those in capsules is y = 1.0264x ? 0.0126 with R2 = 0.9898. Both validation results indicated that the concentrations of aM in MP mixtures were predicted with sufficient accuracy and repeatability. NIR can be a useful tool for the quality control of herbal medicine in powder form without any sample preparation. The type and the shape of the container should be considered to obtain more accurate data. Keywords Near-infrared spectroscopy Garcinia mangostana L. Mangosteen Quantitative analysis Partial least squares
Introduction While modern pharmaceutical drugs are produced from synthetic materials by means of reproducible manufacturing techniques and procedures, herbal medicines are prepared from materials originating from herbs obtained from various geographical and/or commercial sources. Thus, the composition and properties of herbal medicines are assumed to be variable. Good manufacturing practice (GMP) specifies many requirements for the quality control of starting materials of herbal medicines [1]; one of them is quantitation of the amount of active substance. The s
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