Aseptic Peritonitis Model for Drug Discovery (for Prophylaxis)
Infectious or non-infectious peritonitis leads to systemic inflammation due to violation of the peritoneum which is often fatal. Fisetin, a flavonol compound, exhibits a broad spectrum of biological activities including antioxidant, anti-inflammatory, ant
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Aseptic Peritonitis Model for Drug Discovery (for Prophylaxis)
Abbreviations
ASA BM CFU-c DC F Ins LPS MN Cells MTS
MTT MPK NO PB PF PMA PMN Cells PMS PP Spl TC TG
Ascorbic acid Bone marrow Colony-forming units in culture Differential cell count Fisetin Intestine Lipopolysachharide Mononuclear cells [3-(4, 5-dimethyl thiazol-2-yl)-5-(3-carboxy methoxy phenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] 3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide Milligram per kilogram of body weight Nitric oxide Peripheral blood Peritoneal fluid Phorbol 12-myristate 13-acetate Polymorphonuclear cells Phenazine methosulfate Peyer’s patch Spleen Total cell count Thioglycollate
Prophylactic use of fisetin in thioglycollate-induced peritonitis in mice. The original research work included in this chapter has been communicated to BMC Immunology (under revision 8-6-2015). © Springer Science+Business Media Singapore 2016 E. Ray Banerjee, Perspectives in Translational Research in Life Sciences and Biomedicine, DOI 10.1007/978-981-10-0989-1_4
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Aseptic Peritonitis Model for Drug Discovery (for Prophylaxis)
TG3F, TG9F, TG24F
TG3, TG9, TG72, TG24
Treatment with fisetin for 4 days, followed by treatment with 3 % TG 1 h after last fisetin treatment; Kill 3, 9, and 24 h after TG treatment, respectively Treatment with only TG; kill after 3, 9, 24 h, respectively
Symbols
Denotes significance in samples compared to control Denotes significance in samples compared to samples treated with only TG
4.1
Summary of the Original Research
Infectious or non-infectious peritonitis leads to systemic inflammation due to violation of the peritoneum which is often fatal. Fisetin, a flavonol compound, exhibits a broad spectrum of biological activities including antioxidant, anti-inflammatory, anticancer, and neuroprotective effects was used in a murine model of thioglycollate-induced aseptic peritonitis to investigate in, and on RAW macrophage cells. In this study, peritonitis was induced in C57BL/6J mice using thioglycollate, and anti-inflammatory effects of fisetin, was assessed prophylactically. In in vitro study, cells treated with inflammatory agents such as LPS and PMA lose their viability and proliferative capacity. Fisetin has been shown to prevent the loss of viability when given prophylactically. In in vivo model, total cell recruitment was found to increase with TG, showing that it has induced inflammation and interestingly cell recruitment was successfully inhibited by fisetin. The differential count of peripheral blood, treated only with TG, shows an increase in the polymorphonuclear (PMN) cell count, as compared to control. On treatment with fisetin, PMN number decreases. Concentration of nitric oxide (NO) in intestine has increased by 1.90-fold after 3 h (p < 0.05) and 1.24-fold after 24 h (p < 0.05), after treatment with TG as compared to control. NO concentration has decreased by 1.28-fold after 3 h (p < 0.05) and
2.15-fold after 24 h (p < 0.05) with fisetin treatment, compared to only TG. Concentration of as
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