Assessment of IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis by an enzyme-linked immunosorben
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RESEARCH
Open Access
Assessment of IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis by an enzyme-linked immunosorbent assay (ELISA) Renan Marrichi Mauch1, Cláudio Lúcio Rossi1, José Dirceu Ribeiro2, Antônio Fernando Ribeiro2, Marcos Tadeu Nolasco da Silva2 and Carlos Emílio Levy1*
Abstract Background: The usefulness of serological tests for detection of P. aeruginosa pulmonary infection in cystic fibrosis (CF) is controversial. Here, we assessed the value of detecting anti-P. aeruginosa IgG by a quantitative enzyme-linked immunosorbent assay (ELISA) for identification of P. aeruginosa infection in patients with cystic fibrosis. Methods: Serum concentrations of anti-P. aeruginosa IgG were assessed in 117 CF patients classified according to their P. aeruginosa colonization/infection status (never colonized; free of infection; intermittently colonized and chronically infected) and in 53 healthy subjects by the ELISA test standardized with the St-Ag:1–17 antigen. Results: The rate of IgG seropositivity and the median of IgG concentrations of this antibody in patients chronically infected were significantly higher than those found in the other CF groups and in the healthy control group. Conclusion: Detection of anti-P. aeruginosa IgG can be an useful tool for identification of P. aeruginosa chronic infection in patients with CF. Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/ 13000_2014_158 Keywords: Pseudomonas aeruginosa, Cystic Fibrosis, Serology, ELISA
Background Cystic fibrosis (CF) is the most common hereditary disease in the Caucasian population, with a wide range of clinical and genetic variants [1]. The genetic defect occurs in the CF transmembrane conductance regulator (CFTR) gene, which codes for a protein that regulates the transport of electrolytes across epithelial cell membranes. Mutations in the CFTR gene affect sodium and chloride ion transport, resulting in the disruption of the ionic composition and volume of airway surface fluid. This fluid is normally thin to allow removal of inhaled microorganisms via ciliary action; however, in the presence of CFTR mutations, it increases in volume and becomes viscous, clogging the airways. As a result, microorganisms * Correspondence: [email protected] 1 Department of Clinical Pathology, Faculty of Medical Sciences, State University of Campinas, Rua Alexander Fleming 105, Block FCM 12, 2nd floor, Postal code: 13083-881 Campinas, SP, Brazil Full list of author information is available at the end of the article
entering the distal airways are not cleared and can cause chronic infections with progressive inflammation and respiratory insufficiency [2]. The clinical outcomes are similar to those found in diffuse panbronchiolitis, chronic obstructive pulmonary disease and idiopatic pulmonary fibrosis [3-5]. Pseudomonas aeruginosa is an important pathogen in nosocomial and opportunistic infections due to its high intrinsic resistance to antibiotics and ability to de
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