Association of allelic polymorphisms of IGFALS gene with growth traits in Makouei and Ghezel sheep breeds
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Association of allelic polymorphisms of IGFALS gene with growth traits in Makouei and Ghezel sheep breeds Fatemeh Alizadeh 1 & Fatemeh Moradian 1
&
Ayoub Farhadi 2
Received: 21 September 2019 / Accepted: 9 June 2020 # Springer Nature B.V. 2020
Abstract Insulin-like growth factor-binding protein acid-labile subunit (IGFALS) encodes a protein which binds to IGF1 and IGFBP-3 to regulate the growth, differentiation, and other physiological processes. The aim of this study was the identification of allelic polymorphisms of the IGFALS gene using the PCR-RFLP technique and evaluation of their association with growth traits. For this end, 120 blood samples were randomly collected from each breed. Following amplification of an 1113-bp fragment of exon 1 and a part of intron 1 of the IGFLAS gene, genotyping was conducted by three restriction enzymes including HinfI, MscI, and PvuII. The results showed that only one allele was observed in IGFALS-PvuII site, while in IGFLAS-MscI site, three AA, AB, and BB genotypes with the frequencies of 17.5%, 32%, and 50.5% and 11%, 37.5%, and 51.5% were observed in Makouei and Ghezel sheep breeds, respectively. Additionally, in the IGFLAS-HinfI site, two AB and BB genotypes with the frequency of 34.2% and 65.8% were observed in Makouei sheep and AA, AB, and BB genotypes with the frequency of 9%, 21%, and 70% were observed in Ghezel sheep. So that, Makouei sheep with AB genotype had more chest girth (CG) compared with other genotypes. Furthermore, a significant association was observed between the genotypes of IGFLAS-HinfI with birth weight (BW) in Ghezel and BW, weaning weight (BW3), and CG in Makouei sheep. Haplotype analysis revealed an association between paternal haplotypes and BW in both Ghezel and Makouei breeds. So that, AAB and ABB haplotypes showed more BW than others in Makouei and Ghezel sheep, respectively. Keywords IGFALS gene . PCR-RFLP . Growth traits . Makouei sheep . Ghezel sheep
Abbreviations BW Birth weight HW Height of withers CG Chest girth TG Thigh girth BL Body length HR Height of rump WW Wool weight
* Fatemeh Moradian [email protected]; [email protected] 1
Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran
2
Laboratory for Molecular Cytogenetic, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran
Introduction Given the important role of sheep in providing the main part of meat consumption in the country and a high diversity of breeds in Iran as well as the use of wool of this animal in the carpet industry, studying the genes and economic traits in sheep are particularly interested in animal breeding programs. In Iran, sheep meat is more consistent with Iranian people’s tastes and temperament than beef (Valipour-Koutanaee et al. 2019). Nowadays, sheep products have an eminent role in the agricultural economy worldwide (Thornton 2010). The most common sources of meat in Iran are domesticated animal
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