Association of GnRH1 Gene with Growth Traits in Two Breeds of Sheep
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FULL-LENGTH RESEARCH ARTICLE
Association of GnRH1 Gene with Growth Traits in Two Breeds of Sheep Thamer R. S. Aljubouri1
•
Amad F. Hassan2 • Mohammed Baqur S. Al-Shuhaib1
•
Saeid Ansari Mahyari3
Received: 10 January 2020 / Accepted: 31 August 2020 Ó NAAS (National Academy of Agricultural Sciences) 2020
Abstract The present study was conducted to identify the associations between the genetic polymorphisms of GnRH1 gene and growth traits in Awassi and Karakul breeds of sheep. Two GnRH1 amplicons of 313 bp and 323 bp were designed to cover exon 1, exon 2, and their flanking regions, respectively. Genotyping experiments were performed using PCR-SSCP and DNA sequencing. No polymorphism was observed in 323 bp amplicons, while two SSCP-banding patterns were detected in 313 bp amplicons with the presence of two genotypes (AA and AB). Two novel mutations in AB genotype were observed, namely 50 -UTR:50 A [ C and intron1:264 G [ C. In addition to these detected genotypes, results indicated that several growth traits were significantly associated with breed, sex, and dam-age in the first 6 months of birth. However, a tendency of individuals with AA genotype to exert better performance in the majority of measured growth traits than individuals with AB genotype was also observed. With the advent of the month-9 and onward, this tendency developed to be significant (P \ 0.05), in which the association of all growth traits with the GnRH1 genotypes was dominated over other factors. Furthermore, more significant associations were observed in the month-12, in which individuals with AA genotype had higher growth traits measurements than individuals with AB genotype (P \ 0.01). In conclusion, we detected a tight linkage between the polymorphisms of the GnRH1 gene and growth traits at sexual maturity. Therefore, this study strongly suggests the GnRH1 gene as a new candidate in marker-assisted selection for growth traits in sheep. Keywords Awassi Karakul PCR-SSCP SNP Polymorphism Sheep
& Mohammed Baqur S. Al-Shuhaib [email protected]; [email protected] Thamer R. S. Aljubouri [email protected] Amad F. Hassan [email protected] Saeid Ansari Mahyari [email protected] 1
Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, Babil 51001, Iraq
2
Department of Animal Production, College of Agriculture, University of Basrah, Basrah 61001, Iraq
3
Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, PO Box 84156/83111, Isfahan, Iran
Introduction The mammalian ovulation is a complex trait influenced by several genetic and non-genetic factors. Various studies in different sheep breeds showed that one of these genetic factors is the gonadotropin-releasing hormone (GnRH1) gene. It is recently reported that the GnRH1 gene consists of highly conserved sequences among mammalian species [8]. This gene is located in chromosome 2 in the ovine genome and consists of two exons separated by one intron (GenBank acc.
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