Association of molecular markers with physio-biochemical traits related to seed vigour in rice
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RESEARCH ARTICLE
Association of molecular markers with physio-biochemical traits related to seed vigour in rice Swastideepa Sahoo2 • Priyadarsini Sanghamitra1 • Nibedita Nanda2 • Swapnil Pawar1 • Elssa Pandit3 • Ramakrushna Bastia1 • Kumuda Chandra Muduli2 Sharat Kumar Pradhan1
•
Received: 9 April 2020 / Revised: 18 August 2020 / Accepted: 1 September 2020 Ó Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract Eighteen physio-biochemical traits influencing seed vigour were studied for their association with molecular markers using a mini core set constituted from 120 germplasm lines. High genetic variation was detected in the parameters namely chlrophyll a, Chlrophyll b, total chlorophyll, carotenoids, total anthocyanin content, gamma-oryzanols, total phenolics content, superoxide dismutase, catalase, guaicol peroxidase, total soluble sugar, total protein, seed vigour index -I and seed vigour index -II. Strong positive correlation of seed vigour index II was observed with amylose content, total anthocyanin content, catalase, total phenolic content and total flavonoid content while a negative association was observed for gammaoryzanol content. High gene diversity (0.7169) and informative markers value (0.6789) were estimated from the investigation. Three genetic structure groups were observed in the panel population and genotypes were grouped in the subpopulations based on the seed vigour
trait. Differences in the fixation indices of the three sub populations indicated existence of linkage disequilibrium in the studied panel population. Association of the traits namely total flavonoids, superoxide dismutase, catalase, chlorophyll a, Chlorophyll b, total chlorophyll, carotenoids, starch, amylose, total anthocyanin, gamma-oryzanol, total phenolics with the molecular markers were detected by Generalized Linear Model and Mixed Linear Model showing [ 0.10 R2 value. Association of the trait, total flavonoids with marker RM7364 located on chromosome 8 reported in earlier study was validated in this investigation. The validated markers and the novel markers detected showing higher R2 value will be useful for improvement of seed vigour in rice. Keywords Seed vigour Molecular markers Marker-trait association Biochemical traits Physiological traits Rice
Introduction Swastideepa Sahoo and Priyadarsini Sanghamitra have contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12298-020-00879-y) contains supplementary material, which is available to authorized users. & Elssa Pandit [email protected] & Sharat Kumar Pradhan [email protected] 1
Crop Improvement Division, ICAR-National Rice Research Institute, Cuttack, Odisha 753006, India
2
Department of Seed Technology, College of Agriculture, OUAT, Bhubaneswar 751003, Odisha, India
3
FM University, Balasore, Odisha 756020, India
More than half of world population consume rice as staple food not only for calorie needs ‘but also for their nutritional requirement. The
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