Development of EST-SSR markers and association mapping with floral traits in Syringa oblata
- PDF / 1,406,249 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 37 Downloads / 245 Views
RESEARCH ARTICLE
Open Access
Development of EST-SSR markers and association mapping with floral traits in Syringa oblata Yunyao Yang1,2, Ruiqing He2, Jian Zheng2,3, Zenghui Hu1,2,3, Jing Wu1,2,3* and Pingsheng Leng1,2,3
Abstract Background: Lilac (Syringa oblata) is an important woody plant with high ornamental value. However, very limited genetic marker resources are currently available, and little is known about the genetic architecture of important ornamental traits for S. oblata, which is hindering its genetic studies. Therefore, it is of great significance to develop effective molecular markers and understand the genetic architecture of complex floral traits for the genetic research of S. oblata. Results: In this study, a total of 10,988 SSRs were obtained from 9864 unigene sequences with an average of one SSR per 8.13 kb, of which di-nucleotide repeats were the dominant type (32.86%, 3611). A set of 2042 primer pairs were validated, out of which 932 (45.7%) exhibited successful amplifications, and 248 (12.1%) were polymorphic in eight S. oblata individuals. In addition, 30 polymorphic EST-SSR markers were further used to assess the genetic diversity and the population structure of 192 cultivated S. oblata individuals. Two hundred thirty-four alleles were detected, and the PIC values ranged from 0.23 to 0.88 with an average of 0.51, indicating a high level of genetic diversity within this cultivated population. The analysis of population structure showed two major subgroups in the association population. Finally, 20 significant associations were identified involving 17 markers with nine floral traits using the mixed linear model. Moreover, marker SO104, SO695 and SO790 had significant relationship with more than one trait. Conclusion: The results showed newly developed markers were valuable resource and provided powerful tools for genetic breeding of lilac. Beyond that, our study could serve an efficient foundation for further facilitate genetic improvement of floral traits for lilac. Keywords: Syringa oblata, EST-SSR, Genetic diversity, Population structure, Association mapping
Background Lilac, which belongs to the genus Syringa (family Oleaceae), is an important woody ornamental plant. Approximately 27 wild species of Syringa have been described, most of which are native to China [1]. China is the * Correspondence: [email protected] 1 Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture, Beijing 102206, China 2 College of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China Full list of author information is available at the end of the article
world’s distribution center and center of origin for lilac with a cultivation history of 1000 years. After almost 100 years of breeding, there are approximately 2000 lilac cultivars, which show a wide range of forms and colors, and are commonly cultivated throughout the world [2]. S. oblata, a perennial deciduous shrub, is cultivated most widely as a garden plant in northern China
Data Loading...
