Atlas of Human Pluripotent Stem Cells Derivation and Culturing
Human pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, are a key focus of current biomedical research. The emergence of state of the art culturing techniques is promoting the realization of the full potentia
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Series Editor Kursad Turksen, Ph.D. [email protected]
For further volumes: http://www.springer.com/series/7896
Michal Amit
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Joseph Itskovitz-Eldor
Atlas of Human Pluripotent Stem Cells Derivation and Culturing With contributions by Ilana Laevsky, BA and Atara Novak, MSc
Michal Amit, PhD Department of Obstetrics and Gynecology Rambam Health Care Campus Stem Cell Research Center Rapapport Faculty of Medicine Technion – Israel Insitute of Technology Haifa, Israel [email protected]
Joseph Itskovitz-Eldor, MD DSc Department of Obstetrics and Gynecology Rambam Health Care Campus Stem Cell Research Center Rapapport Faculty of Medicine Technion – Israel Insitute of Technology Haifa, Israel [email protected]
ISBN 978-1-61779-547-3 e-ISBN 978-1-61779-548-0 DOI 10.1007/978-1-61779-548-0 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011941608 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface
We are very pleased to present this Atlas on Human Pluripotent Stem Cells— Derivation and Culturing, summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells. The exploration and realization of the incredible potential of these pluripotent stem cells for studying the fundamentals of early human development and organogenesis, for cellbased therapy and for disease modeling in the dish are a key focus of current biomedical research. Although murine embryonic stem cells have been used in research for 4 decades, techniques established for their growth have proven less amenable for long-term culture, expansion and manipulation of human pluripotent stem cells. The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby in 1978 (Steptoe and Edwards 1978) and the discovery in 1981 by Martin (1981) and Evans and Kaufman (1981) of mouse embryonic stem cells. Later, development of sequential media in the mid1990s enabled the growth of fertilized oocytes to viable blastocysts, from which the inner cell mass was extracted and human embryonic st
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