Authentication of camel meat using species-specific PCR and PCR-RFLP
- PDF / 723,813 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 58 Downloads / 184 Views
ORIGINAL ARTICLE
Authentication of camel meat using species-specific PCR and PCR-RFLP S. Vaithiyanathan1 • M. R. Vishnuraj1 • G. Narender Reddy1 • Ch. Srinivas1
Revised: 19 September 2020 / Accepted: 8 October 2020 Ó Association of Food Scientists & Technologists (India) 2020
Abstract In India and some of the African countries, one of the unconventional meats receiving the latest attention in meat adulteration is camel meat. So, the objective of this study was to develop a species-specific PCR based on mitochondrial cytochrome b (CYTB) gene and a PCRRFLP assay of mitochondrial 12S rRNA to identify camel meat in suspected samples. Known sample of camel meat, samples suspected to be from illegally slaughtered camel and known samples of cattle, buffalo, sheep, goat, pork and chicken were used in the study. DNA were extracted from all samples following spin column method and PCR amplification were carried out using both CYTB and 12S rRNA gene primers. The CYTB gene amplification produced amplicon with a size of 435 bp without any nonspecific spurious amplification towards other species studied. Further, the 12S rRNA PCR products were analysed both by sequencing and by RFLP using enzyme AluI. On BLAST analysis the 448 bp sequence obtained from suspected samples showed [ 99% sequence homology to previously reported Camelus dromedaries (accession no: AM 9369251.1). On AluI digestion of the 448 bp product from both known and suspected camel samples, a specific RFLP pattern with three distinct products of 90, 148 and 210 bp size were evident, which were significantly different from the pattern of cattle, sheep, goat, chicken and buffalo. Further, after in-house validation, this cost
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13197-020-04849-w) contains supplementary material, which is available to authorized users. & M. R. Vishnuraj [email protected]; [email protected] 1
ICAR- National Research Centre on Meat, Chengicherla, Hyderabad, Telangana 500092, India
effective and rapid method of camel meat identification is placed into practice for regular screening of vetero-legal samples in the lab. Keywords Camel Meat authentication Speciesspecific PCR-RFLP 12S rRNA CYTB gene Veterolegal
Introduction The ever-increasing demand for meat due to an increasing trend in consumption make the fraudsters to search for unconventional meat, to adulterate with high-quality meat. The driving force behind any adulteration is the revenue maximization, either by using a low-cost ingredient to substitute a more expensive one, or by removing the valued component (Ioannis and Nikolaos 2005). The adulteration of high-value commercial meat, such as mutton, with lowvalue meat, such as chicken, pork and beef are done for financial gains, which results in high motivation of the traders to adulterate (Nischella et al. 2016; Mahajan et al. 2011). In India and some of the African countries, one of the unconventional meats receiving the latest attention of adulteration is cam
Data Loading...
