Basic Confocal Microscopy

Most researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique h

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Robert L. Price  •  W. Gray (Jay) Jerome Editors

Basic Confocal Microscopy

Editors Robert L. Price Department of Cell and   Developmental Biology School of Medicine University of South Carolina 6439 Garner’s Ferry Road Columbia, SC USA

W. Gray (Jay) Jerome Department of Pathology Vanderbilt University Medical Center U-2206 MCN, 1161 21st Avenue, South Nashville, TN USA [email protected]

[email protected]

ISBN 978-0-387-78174-7 e-ISBN 978-0-387-78175-4 DOI 10.1007/978-0-387-78175-4 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011925553 © Springer Science+Business Media, LLC 2011 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)

Preface

Biological confocal microscopy is still a relatively young field. Most researchers in the field would date the modern era of biological confocal microscopy from the 1985 description of a particularly useful confocal design published by White and Amos in the Journal of Cell Biology. Since that time, the use of confocal microscopes by biologists has increased phenomenally, with new converts joining the ranks daily; many with little or no previous microscopy training. For this reason, in 2001 when we were asked to organize a 1 day session on “basic confocal microscopy” for attendees at the Southeastern Microscopy Society annual meeting in Clemson, SC, we decided to focus not only on the confocal microscope itself, but also on ancillary subjects that are critical for getting the most from confocal microscopy. Our initial effort seemed to meet a growing need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. Evidence for this need is that each year since 2001, we have been invited by several meeting organizers and microscopy core facility Directors to present our take on what is important to use confocal microscopy successfully for biological exploration. In 2005, we also