Basic Confocal Microscopy
Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy W
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Basic Confocal Microscopy Second Edition
Basic Confocal Microscopy
W. Gray (Jay) Jerome • Robert L. Price Editors
Basic Confocal Microscopy Second Edition
Editors W. Gray (Jay) Jerome Department of Pathology, Microbiology and Immunology Vanderbilt University School of Medicine Nashville, TN, USA
Robert L. Price Department of Cell Biology and Anatomy University of South Carolina School of Medicine Columbia, SC, USA
ISBN 978-3-319-97453-8 ISBN 978-3-319-97454-5 (eBook) https://doi.org/10.1007/978-3-319-97454-5 Library of Congress Control Number: 2018958885 © Springer Nature Switzerland AG 2011, 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Springer imprint is published by the registered company Springer Nature Switzerland AG The registered company address is: Gewerbestrasse 11, 6330 Cham, Switzerland
Preface
Biological confocal microscopy is still a relatively young and rapidly advancing field. Since the first edition of this book was published in 2011, many significant advances have been made in confocal technology, including the introduction of highly sensitive detectors, improved software applications, several super-resolution instruments based on confocal technology, and more. In preparation of this second edition, it amazed us as to how far the field has advanced in a very short period of time. In this second edition, we introduce many of these advances while attempting to maintain the basic instructional nature of the text. Most chapters have been significantly updated with new information, and an entirely new chapter on analysis of fluorescence co-localization by Dr. Teng-Leong Chew has been added. Most researchers in the field would date the modern era of biological c onfocal microscopy from the 1985 description of a particularly useful confocal design published by White and Amos in the Journal of Cell Biology. Since that
