Biological Applications of Surface Plasmon Resonance Imaging
This chapter will deal with versatile biochips. The presented biochips are prepared by the microarray functionalization of gold surface from millimetric to micrometric scale via different electrochemical approaches. Interactions occurring on these functio
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Abstract This chapter will deal with versatile biochips. The presented biochips are prepared by the microarray functionalization of gold surface from millimetric to micrometric scale via different electrochemical approaches. Interactions occurring on these functionalized surfaces are monitored using surface plasmon resonance imaging approaches. Different size of spots including hyphenated techniques will be used to study biological interactions from molecular (i.e., DNA, proteins (toxins), and sugars) to complex objects such as living cells. Applications of this approach will be illustrated by examples from different biological fields including DNA hybridization studies, antibody screening in patients’ samples, study of oligosaccharides–proteins interactions, and lymphocytes characterization and sorting. For each topic, optimized chemistry and optical developments will be specified. Keywords Biochips • Biological interactions • Polypyrrole • Surface plasmon resonance imaging (SPRi)
Contents 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.1 Chronology of Plasmonic Effect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 Plasmonic Effect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.3 Description of the Technical Approaches Used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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L. Leroy • T. Livache (*) CREAB group, UMR SPrAM CEA/CNRS/ UJF-Grenoble 1, INAC, 17 rue des martyrs, Grenoble 38054, France e-mail: [email protected] E. Maillart Genoptics SA, Horiba scientific, centre R&D, ZI de la vigne aux loups, 5 avenue Arago, ChillyMazarin 91380, France W. Fritzsche and J. Popp (eds.), Optical Nano- and Microsystems for Bioanalytics, Springer Series on Chemical Sensors and Biosensors (2012) 10: 211–226 DOI 10.1007/978-3-642-25498-7_7, # Springer-Verlag Berlin Heidelberg 2012
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2 Biological Applications and Specific Developments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.1 Molecular Interaction Studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.2 Complex Object Studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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1 Introduction Before starting with our main subject, the use of surface plasmon resonance imag
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