Biological Selenium Nano-particles Modify Immune Responses of Macrophages Exposed to Bladder Tumor Antigens
- PDF / 1,299,927 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 1 Downloads / 171 Views
(0123456789().,-volV)(0123456789(). ,- volV)
ORIGINAL PAPER
Biological Selenium Nano-particles Modify Immune Responses of Macrophages Exposed to Bladder Tumor Antigens Pan Liang2
· Deng Qian2 · Guan Zhenfeng2 · Cheng Yongyi2 · Sun Yi2,3 · Liang Liang1
Received: 11 August 2020 / Accepted: 17 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Biological nanoparticles are considered to improve treatment with the least negative complications. Selenium nanoparticles have been used to treat diseases such as pathogenic infections and cancers. In this study, the effect of selenium nanoparticles on macrophage responses was evaluated. Selenium nanoparticles were synthesized biologically by Lactobacillus Plantarum and confirmed through TEM and SEM microscopy, EDX, and XRD characterizations. Macrophage cells were cultured and treated only with selenium nanoparticles and in combination with the bladder tumor lysate. Moreover, tumor lysate only was used as the other group. The genes expression of IFN-γ, IL-27, IL-12, and MHC-I was assessed by real-time PCR method. The production levels of IFN-γ and IL-27 cytokines were evaluated by ELISA assay. Selenium nanoparticles in combination with the tumor lysate increased the genes expression of IFN-γ, IL-27, IL-12, and MHC-I after 24 h and 48 h of treatment. Also, IFN-γ and IL-27 production was increased in the group treated with selenium nanoparticles in combination with the tumor lysate after 48 h and 72 h of treatment. The highest effect of selenium nanoparticles was observed after 48 and 72 h of treatment in the genes expression and cytokines production, respectively. Therefore, the optimum effect of selenium nanoparticle acts as a treatment dependent-manner. Keywords Selenium nanoparticles · Bladder tumor lysate · Macrophage immunity
Introduction The effect of Selenium (Se) as a critical micronutrient ion on biological systems has been reported in various studies. This element plays an important role as an antioxidant, cancer prevention, and preventing cancer through its anticarcinogenic effects [1–4]. Several studies have demonstrated that Se ions can display suppressor effects in different types of tumor cell lines [5, 6]. Some studies have & Sun Yi [email protected] & Liang Liang [email protected] 1
Department of Urology, First Affiliated Hospital of Xi’an Jiaotong University, 277 West Yanta Road, Xi’an 710061, Shaanxi, China
2
Department of Urology, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi, China
3
Department of Urology, Affiliated Hospital of Northwestern Polytechnical University, Xi’an 710068, Shaanxi, China
been conducted on the suppressor anti-tumor effects of organic or inorganic salts of Se ions in mice infected by murine mammary tumor virus [7, 8]. Schrauzer et al. showed that Se ions in both forms of organic and inorganic are effective in decreasing the incidence of breast cancer [7–9]. The biological effects of Se have been reported in the form of selenocysteine followed by metalloid combi
Data Loading...
