Bioremediation of Hydrocarbon Using Bacterial Lipase from Waste Biomass
- PDF / 1,091,753 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 13 Downloads / 185 Views
(0123456789().,-volV) (0123456789().,-volV)
RESEARCH PAPER
Bioremediation of Hydrocarbon Using Bacterial Lipase from Waste Biomass Rajesh Kumar Sahoo1 • Anshuman Sahu1 • Enketeswara Subudhi1 Received: 15 August 2019 / Accepted: 11 July 2020 Ó Shiraz University 2020
Abstract Hydrocarbons originated from automobile oils are significant polluting agents of the soil. Lipase-mediated catalytic degradation of hydrocarbons from this used automobile oil is attempted in the present approach. Lipase was produced from peanut oil industry waste biomass using thermophilic alkaline-tolerant Pseudomonas sp. S1 obtained from sediment of Taptapani hot spring, Odisha. The experiments were designed as per central composite design, which is response surface methodology, to study the effect of the parameters and their interaction on the activity. A quadratic regression was found to be best with the experimental data, R2 = 0.899741 and the maximum lipase production of 51.3 IU/g was predicted from peanut oil seed waste biomass at an optimum pH of 8.31, temperature of 50.58 °C, moisture content of 50.8%, and biosurfactant content of 1.051 mg. The significant interaction of pH and moisture content with biosurfactant during lipase production was observed through the contour plots and 3D surface responses. Pseudomonas sp. S1 could degrade 92.6% of the used automobile oil after incubation for 24 h under optimum pH of 8.31 and temperature of 50.58 °C. Degradation of petroleum hydrocarbons by this thermophilic isolate could be effective due to the ability of lipase to utilize the used automobile oils as sole source of carbon and energy. Keywords Pseudomonas sp. S1 Lipase Peanut oil cake Response surface methodology Solid-state fermentation Hydrocarbon degradation
1 Introduction Polyaromatic hydrocarbons (PAHs) of petrochemical origin have been considered as threat to the environment because of their possible damage to liver or kidney and may be leading to mutagenic and carcinogenic implication (Mishra et al. 2001; Hadibarata and Tachibana 2009; Rubio-Clemente et al. 2014). Used automobile oils, engine oil, diesel oil or jet fuel, generated through emissions due to widespread expansion of motor vehicles, machineries and also jets, have been the primary source of
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40995-020-00935-y) contains supplementary material, which is available to authorized users. & Enketeswara Subudhi [email protected] 1
Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Kalinga Nagar, Bhubaneswar, Odisha 751003, India
contamination (Singh and Lin 2008). The natural habitats of the ecosystem get harmfully affected by them (Husaini et al. 2008). Unlawful discarding of large-scale used oils without following proper majors makes the situation more worsen across the globe (Blodgett 1997). One of the approaches of remediation could be the use of microorganisms to degrade petroleum hydrocarbons as sole source of carbon and energy. Use o
Data Loading...
