Biotechnology of Plant Secondary Metabolism Methods and Protocols
This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolit
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Arthur Germano Fett-Neto Editor
Biotechnology of Plant Secondary Metabolism Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Biotechnology of Plant Secondary Metabolism Methods and Protocols
Edited by
Arthur Germano Fett-Neto Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil
Editor Arthur Germano Fett-Neto Federal University of Rio Grande do Sul Porto Alegre, RS, Brazil
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3391-4 ISBN 978-1-4939-3393-8 (eBook) DOI 10.1007/978-1-4939-3393-8 Library of Congress Control Number: 2015957398 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover Illustration: Seedling of Quillaja brasiliensis (A. St.-Hill. & Tul.) Mart., a tree whose leaves yield immunoadjuvant saponins. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Humans depend on plants not only for food and oxygen but also for medicines, energy, fiber, and building materials. Plant secondary metabolites, in particular, are at center stage as a major source of pharmaceuticals, products for the chemical and food industry, agrochemicals, and special biofuels. Proper manipulation of plant physiology and molecular biology, by means of an array of biotechnological strategies, can promote large gains in yield of desired products. In this book, up-to-date representative methods for improved production of secondary metabolites of economic interest are described in detail, along with tips to avoid common pitfalls in these approaches. An effort was made to include examples applied to the production of different seco
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