Biotransformation of two citrus flavanones by lactic acid bacteria in chemical defined medium
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RESEARCH PAPER
Biotransformation of two citrus flavanones by lactic acid bacteria in chemical defined medium Xiao Guo1 · Ailing Guo1,2 · Erhu Li1,2 Received: 23 August 2019 / Accepted: 26 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Microbial processes are being developed to transform flavonoid glycosides to varieties of metabolites with higher bioavailability. The aim of this study was to determine the metabolic activity and survival of five lactic acid bacteria (LAB) stains (L. rhamnosus LRa05, L. casei LC89, L. plantarum N13, L. acidophilus LA85, and L. brevis LB01) in two different citrus flavanone standards (hesperetin-7-O-rutinoside and naringenin-7-O-rutinoside). The enzymatic activity, metabolites, antioxidant activities, and α-glucosidase inhibition property in the two standards were also investigated before and after incubated with LAB. The medium contained standards permitted survival of the five LAB stains. All strains exhibited β-glucosidase activity. Of the five LAB strains tested, just L. plantarum N13 and L. brevis LB01 have the ability to metabolize hesperetin-7-Orutinoside, only L. plantarum N13, L. acidophilus LA85, and L. brevis LB01 could metabolize naringenin-7-O-rutinoside, moreover, L. acidophilus LA85l was the strain with the highest biotransformation ratio of naringenin-7-O-rutinoside. L. acidophilus LA85 and L. plantarum N13 can degrade naringenin-7-O-rutinoside into naringenin. L. brevis LB01 can degrade hesperetin-7-O-rutinoside into hesperetin, 3-(4′-hydroxyphenyl)-2-propenoic acid, 3-(3′-hydroxy-4′-methoxyphenyl)hydracrylic acid, and 3-(4′-hydroxyphenyl)propionic acid. Incubation of L. acidophilus LA85 in naringenin-7-O-rutinoside solution supposed no apparent influence in the biological activities that tested. L. acidophilus LA85 may potentially contribute to the bioavailability of citrus flavanones, and to be applied as functional cultures to obtain more bioavailable and bioactive metabolites in food products or in the human gastrointestinal tract. Keywords Citrus flavanones · Lactic acid bacteria · Biotransformation · Metabolites · Biological activity
Introduction Flavonoids are very abundant secondary metabolites in plants, which containing two aromatic rings (connected by a heterocycle pyrone ring), and exhibit a common C6-C3-C6 skeleton structure [1, 2]. Prospective studies have reported the anti-inflammatory, antioxidant, anticancer, regulate lipid Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00449-020-02437-y) contains supplementary material, which is available to authorized users. * Erhu Li [email protected] 1
College of Food Science and Technology, Huazhong Agricultural University, 1 Shizishan Road, Wuhan 430070, People’s Republic of China
Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan 430070, Hubei, People’s Republic of China
2
metabolism activities of flavonoids [3–5]. More than 90% of natural
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