Boric-acid-modified Fe 3 O 4 @PDA@UiO-66 for enrichment and detection of glucose by matrix-assisted laser desorption/ion
- PDF / 1,469,325 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 2 Downloads / 118 Views
RESEARCH PAPER
Boric-acid-modified Fe3O4@PDA@UiO-66 for enrichment and detection of glucose by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Zhijian Li 1,2 & Panpan Huo 1 & Congcong Gong 1 & Chunhui Deng 3 & Shouzhi Pu 1 Received: 4 June 2020 / Revised: 11 July 2020 / Accepted: 2 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Herein, boric-acid-modified multifunctional Zr-based metal-organic frameworks (denoted as Fe3O4@PDA@B-UiO-66) were synthesized by hydrothermal reaction and surface modification. Compared to traditional matrix, Fe3O4@PDA@B-UiO-66 has the advantages of high ionization efficiency, high surface area, low matrix background, porous structure, and numerous boricacid-active sites. By combining matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), Fe3O4@PDA@B-UiO-66 was used as an adsorbent and matrix for enrichment and detection of glucose, based on a specific reaction between boric acid and glucose. The limit of detection was 58.5 nM. The proposed method provides a simple and efficient approach for the sensitive and quantitative detection of glucose in complex samples based on MALDI-TOF MS. Keywords Boric acid . Metal-organic framework . MALDI-TOF MS . Enrichment . Glucose
Introduction Diabetes mellitus is a serious metabolic disease associated with abnormal glucose metabolism caused by insulin deficiency or resistance [1]. For patients with diabetes, blood glucose monitoring has extremely important clinical value [2–5]. Over the years, various methods for detecting glucose have been developed, including colorimetry [6–8], surface-enhanced Raman scattering [9, 10], chemiluminescence [11–14], fluorescence [15, 16], electrochemistry [17, 18], and matrixassisted laser desorption/ionization time-of-flight mass * Zhijian Li [email protected] * Chunhui Deng [email protected] * Shouzhi Pu [email protected] 1
Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, Jiangxi, China
2
Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention, Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China
3
Department of Chemistry, Fudan University, Shanghai 200438, China
spectrometry (MALDI-TOF MS). Among these methods, MALDI-TOF MS detection has attracted increasing research interest due to its unique advantages, including high sensitivity, fast response, and low cost. MALDI-TOF MS has been widely applied in various fields for the analysis of proteins [19, 20], nuclear acid polymers [21], and small molecules [22–24]. MALDI-TOF MS works on the mechanism of laser irradiation of a sample incorporated into a UV-absorbing matrix, leading to the desorption and ionization of the sample, which is subsequently detected by TOF-MS [25]. The laser desorption/ionization process and the signal intensity of MALDI-TOF MS usually depend on the matrix. Conventional organic matrices such as 2,5dihydrox
Data Loading...
