Brachypodium Cell Wall Mutant with Enhanced Saccharification Potential Despite Increased Lignin Content

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Brachypodium Cell Wall Mutant with Enhanced Saccharification Potential Despite Increased Lignin Content Hélène Timpano & Richard Sibout & Marie-Françoise Devaux & Camille Alvarado & Rachelle Looten & Xavier Falourd & Bruno Pontoire & Marjolaine Martin & Frédéric Legée & Laurent Cézard & Catherine Lapierre & Eric Badel & Sylvie Citerne & Samantha Vernhettes & Herman Höfte & Fabienne Guillon & Martine Gonneau

Published online: 9 July 2014 # Springer Science+Business Media New York 2014

Abstract Plant lignocellulosic biomass, mostly composed of cell walls, is one of the largest, mostly untapped, reserves of renewable carbon feedstock on the planet. Energy-rich polysaccharide polymers of plant cell walls can be broken down to produce fermentable sugars used to produce bioethanol. However, the complex structure of plant cell walls, and in particular, the presence of lignin, makes them recalcitrant to enzymatic degradation. Reducing this recalcitrance represents a major technological challenge. Brachypodium distachyon is an excellent model to identify parameters underlying biomass quality of energy grasses. In this work, we identified a mutant line spa1 with a so far undescribed phenotype combining Electronic supplementary material The online version of this article (doi:10.1007/s12155-014-9501-1) contains supplementary material, which is available to authorized users. H. Timpano : R. Sibout : M. Martin : F. Legée : L. Cézard : C. Lapierre : S. Citerne : S. Vernhettes : H. Höfte : M. Gonneau INRA, UMR1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, 78000 Versailles, France H. Timpano : R. Sibout : M. Martin : F. Legée : L. Cézard : C. Lapierre : S. Citerne : S. Vernhettes : H. Höfte : M. Gonneau (*) AgroParisTech, Institut Jean-Pierre Bourgin, RD10, 78000 Versailles, France e-mail: [email protected] M.

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