Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibr
- PDF / 1,314,006 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 75 Downloads / 213 Views
RESEARCH ARTICLE
Open Access
Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity Antonio J. Villatoro1,2†, María del Carmen Martín-Astorga1†, Cristina Alcoholado1,3 and José Becerra1,3,4*
Abstract Background: Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results: A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture. Conclusions: Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life. Keywords: Canine colostrum milk, Mesenchymal stem cells, Exosomes, Dog, Anti-oxidative capacity
* Correspondence: [email protected] † Antonio J. Villatoro and María del Carmen Martín-Astorga contributed equally to this work. 1 Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, University of Málaga, IBIMA, 29071 Málaga, Spain 3 Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain Full list of author information is available at the end of the article
Background Canine colostrum milk (CCM) is a specific secretion of the mammary gland produced during the first two days after labour, and it is fundamental for the survival of dogs during the first weeks after birth [1]. In addition to its nutritional function, CCM plays a very significant role in passive immunity, the
© The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 I
Data Loading...
