cDNA Microarrays on Nylon Membranes with Enzyme Colorimetric Detection
Enzyme colorimetric detection is an alternative to fluorescence detection that can be used when simultaneously measuring multiple parameters is required (Tijssen 1985 ; Thorpe and Kerr 1994 ). Colorimetric detection methods combined with the use of microa
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Springer-Verlag Berlin Heidelberg GmbH
B. R. Jordan (Ed.)
DNA Microarrays: Gene Expression Applications With 20 Figures
DR. B.R. JORDAN
Marseille-Genopole Parc Scientifique de Luminy, Case 901 13288 Marseille, Cedex 9 France
ISBN 978-3-540-41508-4
Library of Congress Cataloging-in-PubIication Data DNA microarrays: gene expression appIications I Bertrand Jordan, ed. p. cm Includes bibliographical references and index. ISBN 978-3-540-41508-4 ISBN 978-3-642-56517-5 (eBook) DOI 10.1007/978-3-642-56517-5 1. DNA microarrays-Laboratory manuals. 2. Gene expression-Labordtorymanuals 1. Jordan, Bertrand. QP624.S.D726 D634 2001 572.8'65-dc21 This work is subject to copyright. Ali rights reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law.
http://www.springer.de © Springer-Verlag Berlin Heidelberg 2001 Origina11y published by Springer-Verlag Berlin Heidelberg New York in 2001
The use of general descriptive names, registered names, trademarks, etc. in this publicat ion does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Production: PRO BOIT GmbH, 69126 Heidelberg, Germany Cover design: D&P, 69121 Heidelberg, Germany Typesetting: AM-productions GmbH, 69168 Wiesloch, Germany Printed on acid-free paper SPIN 10792625 39/3130Re - 543210
Preface
The aim of this book is to provide in compact form a comprehensive and practical survey of expression measurement using DNA arrays. I have endeavoured to assemble chapters written by scientists who are actual users of the technology and have had to cope with the various practical problems involved in setting up new methods in the laboratory; I believe this is how such a book can be most useful to its readers. Chapter 1 provides some background on the history of DNA array development; Chapters 2, 3 and 4 focus on various types of micro arrays: glass microarrays are described by experienced academic users, while the less-known (but in some situations superior) nylon micro arrays are presented by their developers together with some information on nylon macroarrays that are still widely used. Chapter 5 describes in detail the use of oligonucleotide chips in a research laboratory, again with emphasis on practical aspects. The principles and practice of both data acquisition and data mining in the field of expression measurement are presented in Chapter 6 by very experienced authors, together with a wealth ofInternet resources
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