Cell biophysical characteristics of PPy-GNPs and their application in photothermal therapy of SKOV-3 cell
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RESEARCH PAPER
Cell biophysical characteristics of PPy-GNPs and their application in photothermal therapy of SKOV-3 cell Chuanzhi Liu & Yuxi Huang & Chunru Zhao & Fan Yang & Haiqi Qin & Zuobin Wang
Received: 16 April 2020 / Accepted: 13 August 2020 # Springer Nature B.V. 2020
Abstract The pursuit of multifunctional, stable, more efficient, and safer cancer treatment has gained increasing interest in the research of nanoparticle-mediated photothermal therapy. Many nanoparticles have good absorption chracteristics in the near-infrared region, because of the high light-to-heat conversion efficiency; they could destroy tumor cells by heat; thus, they are often treated as a potential photothermal therapy agent. In this work, a kind of polypyrrole (PPy)-coated gold nanoparticles particles (PPy-GNPs) with high photothermal conversion capability was prepared. Their particle sizes were about 90 ± 11.2 nm. The temperature of PPy-GNPs solution in a concentration of 0.25 mg/ml rises 13.6 °C, and the nanoparticles could significantly inhibit the proliferation of SKOV-3 cells under irradiation of 25 W·cm−2 laser (808 nm) for 10 min, with the maximum cell viability value is maintained at 87.6% by MTT assay. In the same photothermal and cultured conditions, the photothermal conversion efficiency and cytostatic effect of the gold nanoparticle are much lower than that of the PPy-GNPs group. The atomic force microscopy (AFM) was used to identify the function C. Liu : Y. Huang : C. Zhao : F. Yang : H. Qin : Z. Wang School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China Z. Wang (*) International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China e-mail: [email protected] e-mail: [email protected]
of nanoparticles for the structural changes of the cells. The SKOV-3 cell of adhesion forces decrease by 21%, and Young’s modulus increases by 26% compared with control. These results suggest that PPy-GNPs are effective targeted photothermal therapeutic agents for cancer treatment. Keywords Nanoparticles . Photothermal therapy . SKOV-3 cell . Atomic force microscopy
Introduction Today, nanomaterials are widely used in various fields, especially biomedical engineering. Some studies have shown that different types of nanoparticles (nanoshells, nanorods, nanocages) have a strong capability of killing tumor cells (Torrisi 2015; Marangoni et al. 2016; Connor and Broome 2018). In Mackey et al. (2014) research, they prepared different sizes of nanoparticles. Transmission electron microscopy (TEM) images show that gold nanoparticles (GNPs) encircled the cell and affected vasculature pores. Even more gold nanoparticles are trapped in vesicles in the cytoplasm, which results in higher cytotoxicity and the immunological response of gold nanoparticles, especially those with smaller diameters. When the nanoparticles linked with a marker, they could be recognized in tumors due to an increased expression of the sp
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