Cell Engineering Glycosylation

The analysis and modification of glycans of recombinant proteins continues to be active and challenging area of research and for the successful manufacture of these proteins. In Cell Engineering, volume 3: Glycosylation, Dr. Mohammed Al-Rubeai has compile

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Cell Engineering Volume 3 Editor-in-Chief Dr Mohamed Al-Rubeai School of Chemical Engineering, The University of Birmingham, Edgbaston, Birmingham, U.K. Editorial Board Dr Hansjorg Hauser GBF, Braunschweig, Germany Professor Michael Betenbaugh Johns Hopkins University, Baltimore, U.S.A. Dr. Martin Fusseneger Swiss Federal Institute of Technology Zurich, Switzerland Dr Nigel Jenkins Bioprocess Research & Development, Lilly Research Laboratories, Indianapolis, U.S.A. Professor Caroline MacDonald University of Paisley, Paisley, U.K. Dr Otto-Wilhelm Merten A.F.M.-Genethon 11, Gene Therapy Program, Evry, France

The titles published in this series are listed at the end of this volume.

CELL ENGINEERING Vol. 3: Glycosylation Edited by

Mohamed Al-Rubeai School of Chemical Engineering, The University of Birmingham, Edgbaston, Birmingham, U.K.

KLUWER ACADEMIC PUBLISHERS NEW YORK, BOSTON, DORDRECHT, LONDON, MOSCOW

eBook ISBN: Print ISBN:

0-306-47525-1 1-4020-0733-7

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Glycosylation Cell Engineering Volume 3 With contributions by: Professor Mike Betenbaugh, John Hopkins University, Baltimore, MD, U.S.A. Professor Michael Butler, The Glycobiology Institute, Oxford, U.K. Dr. Harald Conradt, Protein Glycosylation Department, GBF, Braunschweig, Germany Dr. Eckart Grabenhorst, Protein Glycosylation Department, GBF, Braunschweig, Germany Dr. David Harvey, The Glycobiology Institute, Oxford, U.K. Dr. Tsu-An Hsu, National Health Research Institutes, Taipei, Taiwan Professor Roy Jefferis, University of Birmingham, Birmingham, U.K. Dr. Nigel Jenkins, Eli Lilly & Company, Indianapolis, IN, U.S.A. Dr. Lynne Krummen, Genentech, Inc., San Francisco, CA, U.S.A. Dr. S.M. Lawrence, John Hopkins University, Baltimore, MD, U.S.A. Dr. Cheng-Kang Lee, National Taiwan University of Science and Technology, Taipei, Taiwan. Dr. John Lund, University of Birmingham, Birmingham, U.K. Dr. Tony Merry, The Glycobiology Institute, Oxford, U.K. Dr. Lucia Monaco, San Raffaele Scientific Institute, Milan, Italy Dr. Manfred Nimtz, Protein Glycosylation Department, GBF, Braunschweig, Germany Dr. V. Restelli, University of Manitoba, Winnipeg, Manitoba, Canada Dr. Myriam Taverna, Université de Paris Sud, Chatenay-Malabry, France Dr. Thuy Tran, Université de Paris Sud, Chatenay-Malabry, France Dr. Stefanie Weikert, Genentech, Inc., San Francisco, CA, U.S.A. Dr. Jyh-Ming Wu, National Taiwan University of Science and Technology, Taipei, Taiwan. Dr. Kevin Yarema, Johns Hopkins University, Baltimore, U.S.A.

Contents 1

1

ANALYSIS OF GLYCANS OF RECOMBINANT GLYCOPROTEINS T. Merry, M. Taverna, T. T