Cell-Free Protein Expression
Cell-free protein synthesis is coming of age! Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins a
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Cell-Free Protein Expression
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Springer Berlin Heidelberg New York Hong Kong London Milan Paris Tokyo
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James R. Swartz (Ed.)
Ce I-Free Protexpresslon
With 91 Figures and 9 Tables
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Springer
PROFESSOR JAMES R. SWARTZ Department of Chemical Engineering Stanford University Stauffer III, Room 113 381 N. - S. Mall Stanford, CA 94305-5025 USA
ISBN-13:978-3-642-63939-5 Springer Verlag Berlin Heidelberg New York Cataloging-in-Publication Data applied for Bibliographic information published by Die Deutsche Bibliothek. Die Deutsche Bibliothek lists this publication in the Deutsche Nationalbibliografie; detailed bibliographic data is available in the Internet at http://dnb.ddb.de. ISBN -13 :978-3-642-63939-5 e- ISBN -13: 978-3-642-59337-6 DOl: 10.1007/978-3-642-59337-6 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the pro;visions of the German copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law. Springer-Verlag Berlin Heidelberg New York a member of BertelsmannSpringer Science+ Business Media GmbH http://www.springer.de/medizin © Springer-Verlag Berlin Heidelberg 2003
Softcover reprint of the hardcover 1st edition 2003 The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publisher cannot guarantee the accuracy of any information about dosage and application thereof contained in this book. In every individual case the user must check such information by consulting the relevant literature. Cover-Design: design & production GmbH, Heidelberg Typesetting: TypoStudio Tobias Schaedla, Heidelberg Printed on acid-free paper -
SPIN 10921098
18/5141 - 5 4 3 2 1 0
Contents
Part I Template Generation for Cell-Free Protein Expression Systems CHAPTER 1 Generation of Linear Expression Elements by PCR
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C. NEME T Z
2 Rapid Protein Engineering by Expression-PCR C. NEMETZ, S. WESSNER, R. SCHWEITZER, CHAPTER
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A.GRANTZDORFFER,B.BuCHBERGER CHAPTER 3 Expression-PCR: from Gene Pools to Purified Proteins Within 1 Day .... . . . ... . . . . . .... .. .. .. . .. .. .. ..... . .. H . MERK~ D. MESCHKAT, W. STIEGE
4 High-Throughput Expression PCR Used to Systematically Investigate Regulation of Translation Initiation in an E. coli Cell-Free Expression System ... . . ... .. .. ..... . ...... . ..
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CHAPTER
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M. WATZELE, C. NEMETZ, W. OBERMEIER, A. SEIDL, B.BuCHBERGER
5 Reduction of Primer-Dimer Formation during Generation of Expre
