Changes in Complement Levels and Activity of Red Blood Cells, Fresh Frozen Plasma, and Platelet Concentrates During Stor
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ORIGINAL ARTICLE
Changes in Complement Levels and Activity of Red Blood Cells, Fresh Frozen Plasma, and Platelet Concentrates During Storage Xiaomin Liu1 • Ruoshuai Cui1 • Yingwei Song1 • Yang Liu1 • Leiying Zhang1 Shufang Wang1 • Hongfei Zhang1 • Jinghua Sun2 • Ping Di2 • Yang Yu1 • Deqing Wang1
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Received: 14 January 2020 / Accepted: 14 August 2020 Ó Indian Society of Hematology and Blood Transfusion 2020
Abstract Complement cascade plays an important role in the field of transfusion medicine. The study aimed to detect the complement levels of different blood components and different blood types to explore the risk of transfusion of stored blood. The samples including red blood cells (n = 110), fresh frozen plasma (n = 120), and platelet concentrates (n = 104) from healthy blood donors in our center were collected. Complement components (C3, C4, C3b, C3d, and CH50) were assayed to evaluate the activation of complement. The complement levels of various blood components at different storage times were observed. The differences in complement levels of four blood types in various blood components were compared. The complement levels of red blood cells in storage were low, with no significant changes (P [ 0.05). C3b and C3d levels in platelets began to significantly increase after storage for 3 days (P \ 0.05). The fresh frozen plasma during storage had higher complement levels, and the concentrations of C3 and C4 decreased and C3b and C3d increased at month 4 (P \ 0.05). The differences in complement levels of four
Xiaomin Liu and Ruoshuai Cui have contributed equally to the study.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12288-020-01338-0) contains supplementary material, which is available to authorized users. & Yang Yu [email protected] & Deqing Wang [email protected] 1
Department of Blood Transfusion, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China
2
Medical Testing Center, The First Medical Center of Chinese PLA General Hospital, Beijing 100853, China
blood types in various blood components did not significantly change (P [ 0.05), but the C3b and C3d levels of AB fresh frozen plasma remained stable during storage, which different from other blood types. The transfusion of red blood cells was relatively safe in terms of complement activation. The activation of complement proteins occurred during the storage of platelet and plasma, except group AB plasma. Keywords Complement Red blood cells Fresh frozen plasma Platelet Blood type
Introduction There are three pathways for complement activation cascade, of which C3 participates in three pathways and C4 participates in common classical pathways. C3b produced during the activation of C3 can trigger the alternative pathway and eventually cleavage into C3dg, C3c and C3d. The complement cascade plays an important role in the transfusion medicine [1, 2]. In addition, complement is also involved in the development of various diseases. Studies have shown that incom
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