Characterisation of Aceria massalongoi and a histopathological study of the leaf galls induced on chaste trees
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Characterisation of Aceria massalongoi and a histopathological study of the leaf galls induced on chaste trees Enrico de Lillo1 · Elena Fanelli2 · Domenico Valenzano1 · Rosita Monfreda1 · Alberto Troccoli2 · Alessio Vovlas3 · Francesca De Luca2 Received: 10 June 2019 / Accepted: 23 June 2020 / Published online: 1 September 2020 © Springer Nature Switzerland AG 2020
Abstract The eriophyoid mite Aceria massalongoi (Canestrini) was collected from globoid leaf galls on severely injured chaste trees, Vitex agnus-castus L. (Lamiaceae), in Bari and Bernalda (southern Italy), and on the Ionian island Leukade (Greece). Female, male and nymph were described in detail, following the current morphometric descriptive scheme, supplementing older and incomplete descriptions. Molecular characterization of A. massalongoi from Italy and Greece was conducted by amplifying and sequencing the ribosomal ITS, the D2–D3 expansion domains of the 28S rRNA gene and the mitochondrial COI, for the first time. Phylogenetic trees based on the three molecular markers showed congruent results, confirming that Italian and Greek A. massalongoi populations are the same species that cluster together with some intraspecific variability. Galls, ranging from 0.5 to 2.8 mm in diameter, were randomly distributed on both leaf surfaces, and protruded ca. 1 mm from the leaf surface. Sometimes they were closely aggregated on midrib and leaves, which, consequently, appeared strongly deformed. Close-up observations revealed that gall induction causes hyperplastic proliferation of leaf tissues around the gall chamber hosting mites. The uniserial cell lining inside this chamber provides the nutritional tissue for the mites. All feeding cells contained one or more (frequently 2–3) hypertrophied nuclei and dense granular cytoplasm. Keywords Eriophyoidea · Plant feeding mites · Alpha taxonomy · Molecular markers · Gall mite · Chaste tree
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s1049 3-020-00518-x) contains supplementary material, which is available to authorized users. * Enrico de Lillo [email protected] * Francesca De Luca [email protected] Extended author information available on the last page of the article
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Experimental and Applied Acarology (2020) 82:33–57
Introduction Eriophyoidea is a large superfamily of obligate plant-feeding mites, highly host-plant specific with nearly 80% of them found on a single host species, 95% on plant species of the same genus, and 99% on plant species of one family (Skoracka et al. 2010). Aceria (Trombidiformes: Eriophyidae) is the largest known genus among the Eriophyoidea with more than 1000 species spread worldwide (Tajaddod et al. 2018). Aceria massalongoi was described by Canestrini (1890) on leaf samples of the chaste tree, Vitex agnus-castus L. (Lamiaceae, The Plant List 2013), collected in Simeto, Sicily (southern Italy). This species name is the senior homonym of Phyllocoptes massalongoi (Nalepa), which bronzes l
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