Characterization and Partial Sequencing of Calcium Regulating Hormone Stanniocalcin Gene in Freshwater Fish, Labeo rohit
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Characterization and Partial Sequencing of Calcium Regulating Hormone Stanniocalcin Gene in Freshwater Fish, Labeo rohita (Hamilton, 1822) U. Sivagurunathan1,2 • Prem Prakash Srivastava1
Received: 25 March 2020 / Revised: 18 August 2020 / Accepted: 26 August 2020 The National Academy of Sciences, India 2020
Abstract A 421 bp fragment of stanniocalcin gene was amplified in rohu (Labeo rohita). The partially characterized stanniocalcin gene of Labeo rohita is 96% homologous to Sinocyclocheilus rhinocerous stanniocalcin-like mRNA and 95% to Cyprinus carpio stanniocalcin-like mRNA, respectively. The sequence of partially characterized Stanniocalcin was submitted to GenBank (Accession number MF376164) and at present, it is the first report on Indian major carp, rohu (Labeo rohita), which is most popularly cultured fish in aquaculture system in India. Keywords Labeo rohita Corpuscles of Stannius Characterization Stanniocalcin gene Calcium regulating
Stanniocalcin (STC) is a homodimeric glycoprotein hormone involved in calcium and phosphate regulation, a major hypocalcemic factor found in both teleost fish and mammals. Stanniocalcin hormone is synthesized and secreted by small kidney-associated endocrine glands referred to as the corpuscles of Stannius (CS) and also found in some specific regions of the distal renal tubules of basal teleost (Order Osteoglossiformes). Two related mammalian stanniocalcin genes, STC1 and STC2, were found to be expressed in various tissues as paracrine
& Prem Prakash Srivastava [email protected] 1
Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Panch Marg, Versova, Mumbai, 400 061, India
2
Present Address: Aquaculture Research Group (GIA), Universidad de Las Palmas de Gran Canaria (ULPGC), Las Palmas, Canary Islands, Spain
regulators [1]. Although stannniocalcin-1 (STC-1) was originally described in fish, it is now known to be present throughout the animal kingdom in both vertebrates and invertebrates. The morphology of these STC cells was embryologically derived from the pronephric and/or mesonephric ducts and also similar to renal ‘chloride’ (mitochondrial-rich) cells. The number of CS varies from a pair to several hundred in various species [2] from freshwater to marine water. The activation of CS and the release of STC hormone are higher in marine species than that of freshwater species. The changes in their activation are due to the external ionic concentration, and the higher activation of STC hormones is found to be expressed in various regions like the kidney, intestine, gill, and bones. Characterization, expression, and sequencing of STC had been done in several species such as zebrafish [3], salmonids [4], tilapia [5], freshwater and seawater salmon [6], and male and female freshwater teleost Mastacembelus armatus [7]. Hang and Balment [8] studied the expression of euryhaline European flounder (Platichthys flesus) STC in various tissues and organs. They also concluded that the STC mRNA
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