Characterization of Bacillus phage Gxv1, a novel lytic Salasvirus phage isolated from deep-sea seamount sediments
- PDF / 2,203,682 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 58 Downloads / 211 Views
RESEARCH PAPER
Characterization of Bacillus phage Gxv1, a novel lytic Salasvirus phage isolated from deep‑sea seamount sediments Xun Guo1 · Tianyou Zhang1 · Min Jin1,2 · Runying Zeng1,2 Received: 19 May 2020 / Accepted: 14 September 2020 © Ocean University of China 2020
Abstract Seamounts are hotspots for marine life, but to date, no bacteriophages have been reported. Here, a novel Bacillus podophage (named as Bacillus phage Gxv1) was isolated from deep-sea seamount sediments of the western Pacific Ocean (~ 5790 m). Phage Gxv1 has a hexameric head ~ 42–53 nm in diameter and a short tail of ~ 30 nm long, which is a typical feature of the Podoviridae family. One-step curve analysis showed that Gxv1 is a lytic phage that can initiate host lysis within 3.5 h postinfection, and has a relatively large burst size. The 21,781-bp genome contains 34 predicted genes, and the G + C content of phage Gxv1 is 39.69%. Whole-genome comparison of phage Gxv1 with known bacteriophages, using BlastN analysis against the IMG/VR database, revealed that phage Gxv1 is closely related to Bacillus phage phi29 that infects Bacillus subtilis, and their genome-wide similarity is 93.62%. Phylogenetic analysis based on DNA polymerase showed that phage Gxv1 belongs to the Salasvirus genus. Multiple genome alignment showed that phage Gxv1 shares a high level of sequence similarity and common gene order with Bacillus phage phi29. However, some sequences are unique to phage Gxv1, and this region contains genes encoding DNA packing protein, DNA replication protein, and unknown protein. These sequences exhibit low sequence similarity to known bacteriophages, highlighting an unknown origin of these sequences. This study will help improve our understanding of the Salasvirus genus and phage diversity in deep-sea seamounts. Keywords Seamount · Deep sea · Salasvirus · Bacillus phage · Characterization
Introduction Seamounts are isolated topographic elevations with summit depths at least 100 m above the seafloor (Hillier and Watts 2007). Seamounts are one of the most ubiquitous landforms on Earth but are unevenly distributed among ocean basins. As estimated, more than 100,000 seamounts reach a kilometer or more above the seafloor (Wessel et al. 2010), and only a few have been studied extensively. Seamounts are Edited by Jiamei Li. * Min Jin [email protected] * Runying Zeng [email protected] 1
State Key Laboratory Breeding Base of Marine Genetic Resource, Ministry of Natural Resources, Third Institute of Oceanography, Xiamen 361005, China
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China
2
recognized as unique environments and hotspots of biodiversity and biomass (Morato et al. 2010). However, our knowledge of seamount biodiversity is limited. Previous studies have mainly investigated the biodiversity of seamount fauna (Forges et al. 2000; Gollner et al. 2017; Morato et al. 2010), and studies on seamount microorganisms are limited (Ana et al. 2012). In particular, there are only two studies that describe v
Data Loading...
